Prazepam → Nordiazepam
Reaction equation
[CH:1]1[CH:2]=[CH:3][C:4]([C:7]2[C:14]3[CH:15]=[C:16]([Cl:19])[CH:17]=[CH:18][C:13]=3[N:12]([CH2:20]C3CC3)[C:10](=[O:11])[CH2:9][N:8]=2)=[CH:5][CH:6]=1.[OH-].[NH4+]>>[CH3:20][N:12]1[C:10](=[O:11])[CH2:9][N:8]=[C:7]([C:4]2[CH:3]=[CH:2][CH:1]=[CH:6][CH:5]=2)[C:14]2[CH:15]=[C:16]([Cl:19])[CH:17]=[CH:18][C:13]1=2.[CH:1]1[CH:6]=[CH:5][C:4]([C:7]2[C:14]3[CH:15]=[C:16]([Cl:19])[CH:17]=[CH:18][C:13]=3[NH:12][C:10](=[O:11])[CH2:9][N:8]=2)=[CH:3][CH:2]=1Participants
4
Procedure
50 μl of internal standard (15 mg/L prazepam) were added to 1.0 ml of serum sample. Concentrated ammonium hydroxide (0.2 ml) was added prior to extraction with 4.5 ml of n-butyl chloride. The samples were shaken for 5 minutes and then centrifuged. The organic layer was removed and evaporated. The sample was reconstituted in 25 μl ethanol; 2 μl were then injected into the gas chromatograph. Quantitation was done by comparison to standard curves obtained for diazepam and nordiazepam.
Workup
- extractionto extraction with 4.5 ml of n-butyl chloride