Reaction equation
[CH:1]1[CH:6]=[C:5]([N+:7]([O-:9])=[O:8])[C:4]([O:10][C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)=[CH:3][CH:2]=1>C(O)C(N)(CO)CO.Cl>[N+:7]([C:5]1[CH:6]=[CH:1][CH:2]=[CH:3][C:4]=1[OH:10])([O-:9])=[O:8]Participants
3
Reactant 1
Product 1
Reagents, catalysts & solvents 1
Procedure
Cells were harvested in 100 μl of 0,25 M Tris-HCl (pH 8); 80 μl were assayed for CAT activity by liquid scintillation counting using the same procedure as in in vitro translation assays, as described in the Technical Bulletin #084 from Promega, whereas 20 μl was used for assaying the β-galactosidase activity, with a colorimetric assay in which o-nitrophenyl β-D-galacto-side (ONPG) is cleaved to produce o-nitrophenol, as described in a standard protocol (Miller J. H. (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, New-York, pp 352-355).
Sources & evidence
- ORD record
- ord-c6c506226bd64b2baba7b1d0aa29814b
- Source dataset
- uspto-grants-1998_01
- Patent number
- US05707866
Linked notes
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