Tromethamine hydrochloride
Completing data — refresh the page later to see the latest
Basic information
- CAS
1185-53-1- Standard SMILES
Cl.NC(CO)(CO)CO- InChIKey
QKNYBSVHEMOAJP-UHFFFAOYSA-N- Molecular formula
- C4H11NO3.ClH
- Average molar mass
- 157.59 g/mol
- Monoisotopic mass
- 157.05057092
Names & identifiers
- Preferred name
- Tromethamine hydrochloride
- IUPAC
- 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
- English name
- TRIS hydrochloride
- Molecular formula
- C4H12ClNO3
- Molecular weight
- 157.6
Names & synonyms
1185-53-1Tris hydrochlorideTris(hydroxymethyl)aminomethane hydrochlorideTromethamine hydrochloride2-Amino-2-(hydroxymethyl)propane-1,3-diol hydrochlorideTRIS HClTris-HClTrometamol hydrochlorideTrizma hydrochlorideTromethamine HCl1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochlorideTris chloride
Show more names
Tris-HCITRIS-HCL BUFFERTris-HCTRIS-HC1Tris hydrochloric acid
DTXSID4044373Tris hydrochloride bufferUNII-383V75M34EEINECS 214-684-5383V75M34E2-Amino-2-hydroxymethyl-1,3-propanediol hydrochlorideDTXCID2024373TROMETAMOL HYDROCHLORIDE [WHO-DD]
Database identifiers
- CAS
1185-53-1- PubChem CID
- 93573
- DTXSID
DTXSID4044373- ChEMBL
CHEMBL3183206- UNII
- 383V75M34E
- EC Number
- 214-684-5
Properties
Computed structural propertiesFilling in
- Topological polar surface area
- 86.7 Ų
- Hydrogen bond donors
- 5
- Hydrogen bond acceptors
- 4
- Rotatable bonds
- 3
- Heavy atoms
- 9
- Formal charge
- 0
- Complexity
- 54
Experimental & physicochemical properties
Chemical ClassesNitrogen Compounds -> Amino Alcohols, Other
Physical Description
Dry Powder; Liquid; Other Solid; Dry Powder
Hygroscopic solid; [Sigma-Aldrich MSDS]
Physicochemical properties
- Melting point
- 150 · 150-152 °C
- Boiling point
- 225 ℃[at · 225℃[at 101 325 Pa]
- Density
- 1.05 g/mL · 1.05 g/mL at 20 °C
- vapor pressure
- 0 Pa · 0Pa at 20℃
- storage temp.
- room temp
- solubility
- 2 O: · H2O: 4 M at 20 °C, clear, colorless
- form
- crystalline
- color
- clear colorless (40 % (w/w) in H2O) solution
- PH
- 2.5 · 2.5-4.0 (25℃, 4M in H2O)
- pka
- 8.1 · 8.1(at 25℃)
- PH Range
- 7 · 7.0 - 9.0
Safety & regulation
Safety information is for reference only and does not replace the supplier’s Safety Data Sheet (SDS), applicable regulations, or professional assessment.
GHS classification
GHS Classification
This chemical does not meet GHS hazard criteria for 57.2% (103 of 180) of all reports.
Warning
H315 (42.2%): Causes skin irritation [Warning Skin corrosion/irritation]
P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (click each P-code to see the statement)
Aggregated GHS information provided per 180 reports by companies from 16 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.
Hazard information
Regulatory Information
Chemical: 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride
1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1:1) is listed on the EPA's Chemical Data Reporting (CDR) system. Manufacturers and importers of 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1:1) are required to report information about their production and use of this chemical to the EPA under the Toxic Substances Control Act (TSCA). (40 eCFR Part 711)
Status: Active Update: 09-12-2022 https://echa.europa.eu/registration-dossier/-/registered-dossier/19306
2-amino-2-(hydroxymethyl)propane-1,3-diol: Does not have an individual approval but may be used under an appropriate group standard
Hazards SummaryA mild eye irritant in rabbits; Not a sensitizer in experimental animals; [Sigma-Aldrich MSDS] See Tromethamine.
Hazard Classes and CategoriesSkin Irrit. 2 (42.2%)
Safety & protection
Protective Action Criteria (PAC)
Chemistry & industry
Uses & manufacturing
UsesUsed to make surface-active agents and pharmaceuticals, as an emulsifier (for cosmetic creams and lotions, mineral oil, and paraffin wax emulsions), as a biological buffer, and as an alkalizer; [ChemIDplus]
U.S. Production2023: 400,000 - <550,000 lb
Consumer UsesStabilizing agent
Industry UsesOther
General Manufacturing Information
All Other Chemical Product and Preparation Manufacturing
1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1:1): ACTIVE
Applications
DescriptionAbbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0.
UsesTris Hydrochloride is a buffering medium for electrophoresis, molecular biology, and cell culture applications. It may be combined with Tris Base to simplify Tris buffer preparation. Tris Hydrochloride has been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. In immunohistochemistry, Tris Hydrochloride has also been used with urea as a method for antigen retrieval.
General DescriptionTRIS hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.
Preparation methods
The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid.
Related reactions
218 records
Compound notes
Loading…