Tromethamine hydrochloride (HCID 93573)

Names & identifiers

Preferred name: Tromethamine hydrochloride
IUPAC: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
English name: TRIS hydrochloride
Molecular formula: C4H12ClNO3
Molecular weight: 157.6

Names & synonyms

1185-53-1Tris hydrochlorideTris(hydroxymethyl)aminomethane hydrochlorideTromethamine hydrochloride2-Amino-2-(hydroxymethyl)propane-1,3-diol hydrochlorideTRIS HClTris-HClTrometamol hydrochlorideTrizma hydrochlorideTromethamine HCl1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochlorideTris chloride

Show more names

Tris-HCITRIS-HCL BUFFERTris-HCTRIS-HC1Tris hydrochloric acid

DTXSID4044373Tris hydrochloride bufferUNII-383V75M34EEINECS 214-684-5383V75M34E2-Amino-2-hydroxymethyl-1,3-propanediol hydrochlorideDTXCID2024373TROMETAMOL HYDROCHLORIDE [WHO-DD]

Database identifiers

CAS: 1185-53-1
PubChem CID: 93573
DTXSID: DTXSID4044373
ChEMBL: CHEMBL3183206
UNII: 383V75M34E
EC Number: 214-684-5

Properties

Computed structural properties

Topological polar surface area: 86.7 Å²
Hydrogen bond donors: 5
Hydrogen bond acceptors: 4
Rotatable bonds: 3
Heavy atoms: 9
Formal charge: 0
Complexity: 54

Experimental & physicochemical properties

Chemical ClassesNitrogen Compounds -> Amino Alcohols, Other

Physical Description · 2 items

Dry Powder; Liquid; Other Solid; Dry Powder

Hygroscopic solid; [Sigma-Aldrich MSDS]

Physicochemical properties

Melting point: 150 · 150-152 °C
Boiling point: 225 ℃[at · 225℃[at 101 325 Pa]
Density: 1.05 g/mL · 1.05 g/mL at 20 °C
vapor pressure: 0 Pa · 0Pa at 20℃
storage temp.: room temp
solubility: 2 O: · H2O: 4 M at 20 °C, clear, colorless
form: crystalline
color: clear colorless (40 % (w/w) in H2O) solution
PH: 2.5 · 2.5-4.0 (25℃, 4M in H2O)
pka: 8.1 · 8.1(at 25℃)
PH Range: 7 · 7.0 - 9.0
biological source: synthetic
Water Solubility: SOLUBLE
Sensitive: Hygroscopic
λmax: 260 nm · λ: 260 nm Amax: 0.12λ: 280 nm Amax: 0.08
BRN: 3675235 · 3675235
Stability: Stable. Incompatible with strong oxidizing agents, bases, moisture.
Cosmetics Ingredients Functions: ANTIMICROBIAL
InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChIKey: QKNYBSVHEMOAJP-UHFFFAOYSA-N
SMILES: Cl.NC(CO)(CO)CO
LogP: -3.6 at · -3.6 at 20℃
CAS DataBase Reference: 1185 · 1185-53-1(CAS DataBase Reference)
FDA UNII: 383 V75M34E · 383V75M34E
EPA Substance Registry System: 1 · 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1185-53-1)
Absorption: 0.05 at · ≤0.05 at 290nm at 40%
UNSPSC Code: 51102829 · 51102829
NACRES: 25 · NA.25
Symbol(GHS): 7 · GHS07
Signal word: Warning
Hazard statements: 315 · H315-H319-H335
Precautionary statements: 305 · P305+P351+P338
Hazard Codes: Xi
Risk Statements: 36 · 36/37/38
Safety Statements: 26 · 26-36
WGK Germany: 3 · 3
RTECS: 2900000 · TY2900000
F: 3 · 3
TSCA: TSCA listed
HS Code: 29221980 · 29221980
Storage Class: 11 · 11 - Combustible Solids
REACH Registrations: Active

Safety & regulation

GHS classification

GHS Classification · 5 items

This chemical does not meet GHS hazard criteria for 57.2% (103 of 180) of all reports.

Warning

H315 (42.2%): Causes skin irritation [Warning Skin corrosion/irritation]

P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (click each P-code to see the statement)

Aggregated GHS information provided per 180 reports by companies from 16 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.

Hazard information

Regulatory Information · 4 items

Chemical: 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride

1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1:1) is listed on the EPA's Chemical Data Reporting (CDR) system. Manufacturers and importers of 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1:1) are required to report information about their production and use of this chemical to the EPA under the Toxic Substances Control Act (TSCA). (40 eCFR Part 711)

Status: Active Update: 09-12-2022 https://echa.europa.eu/registration-dossier/-/registered-dossier/19306

2-amino-2-(hydroxymethyl)propane-1,3-diol: Does not have an individual approval but may be used under an appropriate group standard

Hazards SummaryA mild eye irritant in rabbits; Not a sensitizer in experimental animals; [Sigma-Aldrich MSDS] See Tromethamine.

Hazard Classes and CategoriesSkin Irrit. 2 (42.2%)

Safety & protection

Protective Action Criteria (PAC) · 3 items

18 [mg/m3]

190 [mg/m3]

1200 [mg/m3]

Safety data

F: 3
wgk: 3
tsca: TSCA listed
rtecs: TY2900000
hs_code: 29221980
risk_code: 36/37/38
Signal word: Warning
Symbol(GHS): GHS07
hazard_code: Xi
safety_code: 26-36
Storage Class: 11 - Combustible Solids
Hazard statements: H315-H319-H335
REACH Registrations: Active
Precautionary statements: P305+P351+P338

Chemistry & industry

Uses & manufacturing

UsesUsed to make surface-active agents and pharmaceuticals, as an emulsifier (for cosmetic creams and lotions, mineral oil, and paraffin wax emulsions), as a biological buffer, and as an alkalizer; [ChemIDplus]

U.S. Production2023: 400,000 - <550,000 lb

Consumer UsesStabilizing agent

Industry UsesOther

General Manufacturing Information · 2 items

All Other Chemical Product and Preparation Manufacturing

1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1:1): ACTIVE

Applications · 3 items

DescriptionAbbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0.

UsesTris Hydrochloride is a buffering medium for electrophoresis, molecular biology, and cell culture applications. It may be combined with Tris Base to simplify Tris buffer preparation. Tris Hydrochloride has been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. In immunohistochemistry, Tris Hydrochloride has also been used with urea as a method for antigen retrieval.

General DescriptionTRIS hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Preparation methods · 1 item

PreparationThe preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid.