Platinum → HCID 178443123, compound

HRID 841004

Reaction equation

HRID 841004 reaction equation
[Pt:1].[C:2]([O:19]C(=O)CCCCCCCCCCCCCCC)(=O)[CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH3:17]>C(=O)(O)[O-].C([NH+](CC)CC)C.C(O)(C)C.O.[Pd]>[C:2]([Pt:1])(=[O:19])[CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH3:17]

Participants

6

Reactant 2

HCID 23939PlatinumHCID 69339Palmitic anhydride

Product 1

HCID 178443123HCID 178443123, compound

Reagents, catalysts & solvents 3

HCID 23938PalladiumCatalystHCID 11768884Triethylammonium hydrogen carbonateSolventHCID 21889133Propan-2-ol--water (1/1)Solvent

Procedure

Preparation used the same procedure as the platinum cluster conjugate: an estimated 30 nmol of amino-derivatized 2-3 nm palladium cluster, dissolved in 0.6 M triethylammonium bicarbonate in 20% isopropanol/water, was evaporated to dryness five times from methanol, dissolved in 3 mL of dichloromethane, then treated with 200 nmol of palmitic anhydride. The mixture was stirred for one hour at room temperature, washed three times with 0.1 M sodium phosphate buffer at pH 6.5, evaporated to dryness, redissolved in 2:1 methanol/chloroform and separated by gel filtration (GH25, Amicon, eluted with 2:1 methanol:chloroform). After evaporation to dryness, lipid conjugation was demonstrated by extraction of the cluster label into the chloroform layer when the palmitoyl palladium cluster conjugate was shaken in a chloroform/water mixture with a small amount of methanol as a phase transfer agent.

Workup

  1. customPreparation
  2. customwas evaporated to dryness five times from methanol
  3. dissolutiondissolved in 3 mL of dichloromethane
  4. washwashed three times with 0.1 M sodium phosphate buffer at pH 6.5
  5. customevaporated to dryness
  6. dissolutionredissolved in 2:1 methanol/chloroform
  7. customseparated by gel filtration (GH25, Amicon
  8. washeluted with 2:1 methanol
  9. customAfter evaporation to dryness, lipid conjugation
  10. extractionwas demonstrated by extraction of the cluster label into the chloroform layer when the palmitoyl palladium cluster conjugate
  11. stirringwas shaken in a chloroform/water mixture with a small amount of methanol as a phase transfer agent

Sources & evidence

ORD record
ord-82b5e0c06bf94248b0ea42ee77367dc3
Source dataset
uspto-grants-2002_04
Patent number
US06369206B1

Linked notes

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