Sulfuric acid magnesium salt (1:1) → D-(-)-Fructose
Reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].[O-]S([O-])(=O)=O.[Mg+2].C(O)C(N)(CO)CO.C([O-])(=O)/C=C\C([O-])=O>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]Participants
4
Conditions
- Temperature
- 85 °C
Procedure
The conditions used for the bioconversion are the following: To a 5 ml solution of 0.8 M glucose, 10 mM MgSO4, 1 mM CoCl2, 50 mM Tris/maleate pH 6.4 was added 200 μl of the enzyme preparation. The temperature is maintained at 85° C. for 120 min to effectuate the bioconversion. Subsequently, the amount of fructose formed is determined chromatographically by HPLC.