2-Oxoglutaric Acid → cis-3-Hydroxy-L-proline

HRID 848959

Reaction equation

HRID 848959 reaction equation
[NH:1]1[CH2:8][CH2:7][CH2:6][C@H:2]1[C:3]([OH:5])=[O:4].[O:9]=C(CCC(O)=O)C(O)=O.O=C1O[C@H]([C@H](CO)O)C(O)=C1O>>[OH:9][C@@H:6]1[CH2:7][CH2:8][NH:1][C@@H:2]1[C:3]([OH:5])=[O:4]

Participants

4

Reactant 3

HCID 512-Oxoglutaric AcidHCID 145742L-ProlineHCID 54670067L-Ascorbic Acid

Product 1

HCID 7098652cis-3-Hydroxy-L-proline

Conditions

Temperature
100 °C

Procedure

The enzyme preparation to be determined is added to 100 mM TES buffer (pH 7.0) containing 5 mM L-proline, 5 mM 2-ketoglutaric acid, 1 mM ferrous sulfate and 5 mM L-ascorbic acid to make 100 μl in total, and the mixture was allowed to stand at 35° C. for 10 minutes. The reaction mixture is heated at 100° C. for 2 minutes so as to stop the reaction, and the amount of cis-3-hydroxy-L-proline produced in the reaction mixture is determined by high performance liquid chromatography (hereinafter referred to as HPLC).

Workup

  1. customThe enzyme preparation
  2. additionto be determined is added to 100 mM TES buffer (pH 7.0)
  3. customthe reaction

Sources & evidence

ORD record
ord-403476d414564f0992b50f896ab7cf8a
Source dataset
uspto-grants-2000_01
Patent number
US06010891

Linked notes

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