NHS-Biotin → Biotin

HRID 870120Notes

Reaction equation

HRID 870120 reaction equation
C1C(=O)N([O:8][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][CH:15]2[S:19][CH2:18][CH:17]3[NH:20][C:21]([NH:23][CH:16]23)=[O:22])=[O:10])C(=O)C1>CN(C=O)C>[OH:10][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][C@H:15]1[C@@H:16]2[C@@H:17]([NH:20][C:21]([NH:23]2)=[O:22])[CH2:18][S:19]1)=[O:8]

Participants

3

Reactant 1

HCID 434213NHS-Biotin2×

Product 1

HCID 171548Biotin

Reagents, catalysts & solvents 1

HCID 6228DimethylformamideSolvent2×

Procedure

Amine functionalized polymer of formula (Vc) is dissolved at 10 mg/mL in anhydrous DMF and divided into two portions. NHS-biotin (0.9 mg in 90 μL, 88 equivalents) (Pierce, 20217) and NHS-LC-LC-biotin (Pierce, 21343) at 10 mg/mL (1.5 mg in 150 μL, 88 equivalents) are dissolved in anhydrous DMF. The NHS-biotin and NHS-LC-LC-biotin solutions are immediately added to the two portions of polymer solution and allowed to mix on a shaker overnight in the dark.

Workup

  1. additionto mix on a shaker overnight in the dark

Sources & evidence

ORD record
ord-3d38da48bf4b438283170f9d02992ad6
Source dataset
uspto-grants-2015_03
Patent number
US08969509B2

Reaction notes

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