Sodium Hydroxide → HCID 178482882, compound

HRID 921977

Reaction equation

HRID 921977 reaction equation
[CH3:1][N:2]([C:4]1[C:9]2[CH2:10][C@@H:11]3[C:21]([C:22](=[O:23])[C:8]=2[C:7]([OH:33])=[CH:6][CH:5]=1)=[C:20]([OH:24])[C@@:19]1([OH:25])[C@H:13]([C@H:14]([N:30]([CH3:32])[CH3:31])[C:15]([OH:29])=[C:16]([C:26]([NH2:28])=[O:27])[C:17]1=[O:18])[CH2:12]3)[CH3:3].[Cl-:34].[Cl-].[Ca+2].C[C@@H]1[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@@]2(O)O[C@H]([C@H](C(O)=O)[C@@H](O)C2)C[C@@H](O[C@@H]2O[C@H](C)[C@@H](O)[C@H](N)[C@@H]2O)C=CC=CC=CC=CC=CC=CC=C1.[OH-].[Na+]>[Mg+2].[Cl-].[Cl-]>[CH3:3][N:2]([C:4]1[C:9]2[CH2:10][C@@H:11]3[C:21]([C:22](=[O:23])[C:8]=2[C:7]([OH:33])=[CH:6][CH:5]=1)=[C:20]([OH:24])[C@@:19]1([OH:25])[C@H:13]([C@H:14]([N:30]([CH3:32])[CH3:31])[C:15]([OH:29])=[C:16]([C:26]([NH2:28])=[O:27])[C:17]1=[O:18])[CH2:12]3)[CH3:1].[ClH:34]

Participants

6

Reactant 4

HCID 14798Sodium HydroxideHCID 5280965Amphotericin BHCID 5284359Calcium ChlorideHCID 178482881HCID 178482881, compound2×

Product 1

HCID 178482882HCID 178482882, compound

Reagents, catalysts & solvents 1

HCID 5360315Magnesium ChlorideSolvent2×

Procedure

In vitro hemolysis of rabbit red blood cells (RBCs) after exposure to minocycline formulated in MgCl2 or CaCl2 was compared to in vitro hemolysis of RBCs after exposure to minocycline in saline, or exposure to amphotericin B. Minocycline HCl (LKT laboratories) stock solutions were prepared with MgCl2 in saline, saline, or lactated ringer, and the pH was adjusted with NaOH. Rabbit and sheep red blood cells (RBCs) were obtained from Innovative Research laboratory (Michigan, USA). Immediately before use, RBCs were washed three times in 0.9% saline and adjusted to a density of 5% in saline. 200 μl RBCs was added to 800 μl minocycline solution, and mixed by gentle inversion for 2-5 seconds. Samples were incubated at 37° C. or 30 minutes or at 25° C. for 2-5 minutes. Incubated samples were centrifuged at 12000×g for 4 minutes and the supernatants were removed and the hemoglobin absorbance was read at 540 nm. Samples were tested in triplicate. Amphotericin B (MP Biomedicals) and distilled H2O, or Triton-x and distilled H2O were used as positive controls; saline was used as a negative control. Percent hemolysis was calculated according to the following formula:

Workup

  1. customRabbit and sheep red blood cells (RBCs) were obtained from Innovative Research laboratory (Michigan, USA)
  2. washImmediately before use, RBCs were washed three times in 0.9% saline
  3. addition200 μl RBCs was added to 800 μl minocycline solution
  4. additionmixed by gentle inversion for 2-5 seconds
  5. waitg for 4 minutes
  6. customthe supernatants were removed
  7. distillationAmphotericin B (MP Biomedicals) and distilled H2O, or Triton-x
  8. distillationdistilled H2O

Sources & evidence

ORD record
ord-f7fead0385774ea8b19f26ea69fe80bd
Source dataset
uspto-grants-2016_03
Patent number
US09278105B2

Linked notes

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