(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → beta-Maltose

HRID 981011

Reaction equation

[CH2:1]([OH:45])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@@H:12]([O:17][C@H]3[C@H](O)[C@@H](O)[C@@H](O[C@H]4[C@H](O)[C@@H](O)[C@@H](O)O[C@@H]4CO)O[C@@H]3CO)[O:11][C@@H:10]2[CH2:40][OH:41])[C@H:5]([OH:42])[C@@H:4]([OH:43])[C@@H:3]1[OH:44].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O>>[CH2:1]([OH:45])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@H:12]([OH:17])[O:11][C@@H:10]2[CH2:40][OH:41])[C@H:5]([OH:42])[C@@H:4]([OH:43])[C@@H:3]1[OH:44]

Participants

Reactant 2

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HCID 446495Alpha-maltotetraose4×

Product 1

HCID 6255beta-Maltose

Procedure

Maltotetraose is the preferred α(1→4) linked glucan for use in the present invention. Maltotetraose is an oligosaccharide consisting of four α(1→4) linked glucose units and is commercially available from Sigma Chemical Company, St. Louis, Missouri, or Boehringer Mannheim, GmbH, Mannheim, West Germany. Where maltotetraose is employed as the α(1→4) linked glucan, the kinetic assay proceeds as follows: Maltotetraose substrate is hydrolyzed by α-amylase in the test sample to yield two moles of maltose per mole of substrate. The enzyme maltose phosphorylase (MP) catalyzes the phosphorolysis of each mole of maltose to one mole each of glucose and β-D-glucose-1-phosphate. The enzyme β-phosphoglucomutase (β-PGM) converts the β-glucose-1-phosphate to glucose-6-phosphate which is then oxidized to 6-phosphogluconate with the concomitant reduction of nicotinamide adenine dinucleotide (NAD) to NADH in the reaction catalyzed by G-6-PDH.

Sources & evidence

ORD record: ord-0d4e50c39d4f4a61a99705fec6be2718
Source dataset: uspto-grants-1991_02
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US04990445

Linked notes

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