α-D-グルコピラノース-1-ホスファート

解説glucose 1-dihydrogen phosphate．C6H13O9P(260.14)．動物，植物中に広く存在する．UDP-グルコースを介して，植物ではデンプン生成の前駆体として，動物ではグリコーゲンの前駆体として重要な化合物であり，また同時に，デンプンやグリコーゲンの解糖過程の初期生成物でもある．筋肉中から得られ，化学的には，α-アセトブロモグルコースとジフェニルホスファートの銀塩から合成される．"[α]25D＋120°(水)．バリウム塩，カリウム塩は安定である．正リン酸より酸性は強い(pK1 1.11，pK2 6.13)．[CAS 59-56-3]森北出版「化学辞典（第2版）

純化方法Two litres of a 5% aqueous solution of the phosphate are purified by adjusting the pH to 3.5 with glacial acetic acid (+ 3g of charcoal) and filtering. An equal volume of EtOH is added, the pH is adjusted to 8.0 (glass electrode) and the solution is stored at 3o overnight. The precipitate is filtered off, dissolved in 1.2L of distilled water, filtered and an equal volume of EtOH is added. After standing at 0o overnight, the crystals are collected at the centrifuge and washed with 95% EtOH, then absolute EtOH, ethanol/diethyl ether (1:1), and diethyl ether. [Sutherland & Wosilait, J Biol Chem 218 459 1956.] Its barium salt can be crystallised from water and EtOH. Heavy metal impurities are removed by passage of an aqueous solution (ca 1%) through an Amberlite IR-120 column (in the appropriate H+, Na+ or K+ forms). Di-K salt crystallises as a dihydrate from EtOH. [see McGready Biochemical Preparations 4 63 1955.] [Beilstein 17/8 V 247.]