Udp-Glucose → 3'-Azido-2',3'-Dideoxyuridine 5-O-Diphosphoglucose

HRID 467265

反応式

HRID 467265 の反応式
[CH2:1]1[C@H:5]([N:6]2[C:12](=[O:13])[NH:11][C:9](=[O:10])[CH:8]=[CH:7]2)[O:4][C@H:3]([CH2:14][OH:15])[C@H:2]1[N:16]=[N+:17]=[N-:18].[O-:19][P:20]([O:23][P:24]([O:27]P([O-])([O-])=O)([O-:26])=[O:25])(=[O:22])[O-:21].[CH2:32]([OH:47])[C@H:33]1[O:38][C@H:37](OP(O)(O)=O)[C@H:36]([OH:44])[C@@H:35]([OH:45])[C@@H:34]1[OH:46].[Mg+2].[Cl-].[Cl-].C(O)C(N)(CO)CO.Cl.C1C(=O)NC(=O)N([C@@H]2O[C@H](COP(OP(O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)(O)=O)(O)=O)[C@@H](O)[C@H]2O)C=1.Cl(O)(=O)(=O)=O>>[N:16]([C@@H:2]1[C@@H:3]([CH2:14][OH:15])[O:4][C@@H:5]([N:6]2[CH:7]=[CH:8][C:9](=[O:10])[NH:11][C:12]2=[O:13])[CH2:1]1)=[N+:17]=[N-:18].[P:24]([O:27][C@H:33]([CH2:32][OH:47])[C@@H:34]([OH:46])[C@H:35]([OH:45])[C@@H:36]([OH:44])[CH:37]=[O:38])([O:23][P:20]([OH:21])([OH:19])=[O:22])([OH:26])=[O:25]

参加物

7

反応物 6

HCID 8629Udp-GlucoseHCID 24247Perchloric AcidHCID 65533alpha-D-glucose 1-phosphateHCID 93573Tromethamine hydrochlorideHCID 5360315Magnesium ChlorideHCID 131717599AzddU triphosphate

生成物 1

HCID 1317175983'-Azido-2',3'-Dideoxyuridine 5-O-Diphosphoglucose

実験手順

To 150 milligrams of AzddU-triphosphate was added 400 milligrams of glucose-1-phosphate, 6 millimole of MgCl2, 0.70 millimole of Tris-HCl pH 7.6, bacterial UDPG-pyrophosphorylase and inorganic pyrophosphatase. After 4 hours at room temperature, the reaction was stopped with perchloric acid (7N). After 30 minutes in ice, samples were centrifuged and the supernatant was neutralized with base. After another 30 minutes in ice, samples were recentrifuged and AzddU-5'-diphosphoglucose was purified by column chromatography.

後処理

  1. waitAfter 30 minutes in ice
  2. waitAfter another 30 minutes in ice
  3. customAzddU-5'-diphosphoglucose was purified by column chromatography

出典と根拠

ORDレコード
ord-3cfdeae288a2409fb130096d830f42b3
出典データセット
uspto-grants-1992_10
特許番号
US05159067

関連ノート

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