Urea → 2-keto-L-Gulonic acid

HRID 1033722

反応式

HRID 1033722 の反応式
[OH:1][CH2:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].NC(N)=[O:15].OP([O-])(O)=O.[K+].C([O-])([O-])=O.[Ca+2]>>[CH2:2]([OH:1])[C@H:3]([OH:4])[C@@H:5]([OH:6])[C@H:7]([OH:8])[C:9]([C:11]([OH:15])=[O:12])=[O:10]

参加物

5

反応物 4

HCID 1176UreaHCID 5780SorbitolHCID 10112Calcium CarbonateHCID 516951Monobasic potassium phosphate

生成物 1

HCID 1024242-keto-L-Gulonic acid

実験手順

Five ml of the resulting individual seed cultures as prepared above was inoculated into a 500 ml of Erlenmeyer flask containing 50 ml of production medium (PM): D-sorbitol 8%, corn steep liquor 1%, urea 1.5% (sterilized separately), KH2PO4 0.1%, MgSO4.7H2O 0.01%, CaCO3 0.6% and antifoam 0.1% (pH 7.0 before sterilization) and incubated at 30° C. for 4 days with shaking (180 rpm). Table 1 summarizes the results of the quantitative determination of 2-keto-L-gulonic acid of the cultured broths as assayed by high performance liquid chromatography.

後処理

  1. customFive ml of the resulting individual seed cultures as prepared
  2. additioncontaining 50 ml of production medium (PM)

出典と根拠

ORDレコード
ord-5749d793ee5347d998bda00a14d9639e
出典データセット
uspto-grants-1994_05
特許番号
US05312741

関連ノート

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