Diisopropylethylamine → 2-(4-bromophenyl)-5-cyclopropyl-N-methyl-6-[(methylsulfonyl){2-[(2H-tetrazol-5-ylacetyl)amino]ethyl}amino]-2H-indazole-3-carboxamide

HRID 1037548ノート

反応式

HRID 1037548 の反応式
[NH2:1][CH2:2][CH2:3][N:4]([S:28]([CH3:31])(=[O:30])=[O:29])[C:5]1[C:6]([CH:25]2[CH2:27][CH2:26]2)=[CH:7][C:8]2[C:12]([CH:13]=1)=[N:11][N:10]([C:14]1[CH:19]=[CH:18][C:17]([Br:20])=[CH:16][CH:15]=1)[C:9]=2[C:21]([NH:23][CH3:24])=[O:22].[NH:32]1[C:36]([CH2:37][C:38](O)=[O:39])=[N:35][N:34]=[N:33]1.CN(C(ON1N=NC2C=CC=NC1=2)=[N+](C)C)C.F[P-](F)(F)(F)(F)F.CCN(C(C)C)C(C)C>CN(C=O)C.C(OCC)(=O)C>[Br:20][C:17]1[CH:16]=[CH:15][C:14]([N:10]2[C:9]([C:21]([NH:23][CH3:24])=[O:22])=[C:8]3[C:12]([CH:13]=[C:5]([N:4]([S:28]([CH3:31])(=[O:29])=[O:30])[CH2:3][CH2:2][NH:1][C:38](=[O:39])[CH2:37][C:36]4[N:32]=[N:33][NH:34][N:35]=4)[C:6]([CH:25]4[CH2:26][CH2:27]4)=[CH:7]3)=[N:11]2)=[CH:19][CH:18]=1

参加物

7

反応物 4

HCID 81531DiisopropylethylamineHCID 9886157Methanaminium, N-((dimethylamino)(3H-1,2,3-triazolo(4,5-b)pyridin-3-yloxy)methylene)-N-methyl-, hexafluorophosphate(1-) (1:1)HCID 569627726-[(2-aminoethyl)(methylsulfonyl)amino]-2-(4-bromophenyl)-5-cyclopropyl-N-methyl-2H-indazole-3-carboxamideHCID 178525261HCID 178525261、化合物

生成物 1

HCID 569628452-(4-bromophenyl)-5-cyclopropyl-N-methyl-6-[(methylsulfonyl){2-[(2H-tetrazol-5-ylacetyl)amino]ethyl}amino]-2H-indazole-3-carboxamide収率 41.2%

試薬・触媒・溶媒 2

HCID 6228Dimethylformamide溶媒HCID 8857Ethyl Acetate溶媒

実験手順

To a mixture of Compound (7) (34 mg, 67 μmol) and tetrazole acetic acid (10 mg, 81 μmol) in DMF (3 mL) at 0° C. was added HATU (31 mg, 81 μmol) and DIPEA (18 μL, 0.1 mmol). The mixture was stirred at RT for 40 min. Ethyl acetate (5 mL) was added and the mixture was washed with 1M hydrochloric acid and saturated sodium bicarbonate. The combined aqueous phase was neutralised to pH 7 (by addition of 6M hydrochloric acid) and lyophilised. The crude material was then purified by reverse phase flash column chromatography eluting with ACN/water (5-100%) to give Compound (8) (17 mg, 41%). ESI-MS m/z calculated for [M+H]+: 616.11/618.11; found: 615.96/617.96. 1H NMR (400 MHz, d4-MeOH) δ 9.42 (s, 1H), 9.27 (d, J=8.7 Hz, 2H), 9.10 (d, J=8.7 Hz, 2H), 8.87 (s, 1H), 5.55-5.37 (m, 2H), 5.37-5.14 (m, 2H), 5.01 (t, J=6.5 Hz, 2H), 4.70 (s, 3H), 4.52 (s, 3H), 3.95-3.73 (m, 1H), 2.69-2.41 (m, 3H), 2.27-2.10 (m, 1H).

後処理

  1. washthe mixture was washed with 1M hydrochloric acid and saturated sodium bicarbonate
  2. additionThe combined aqueous phase was neutralised to pH 7 (by addition of 6M hydrochloric acid)
  3. customThe crude material was then purified by reverse phase flash column chromatography
  4. washeluting with ACN/water (5-100%)

出典と根拠

ORDレコード
ord-396c00d7e8394a0b94c7c91b13a3fc7d
出典データセット
uspto-grants-2013_10
特許番号
US08546389B2

反応ノート

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