Thymine → 5-Methyluridine

HRID 1078177

反応式

HRID 1078177 の反応式
[CH3:1][C:2]1[C:3](=[O:9])[NH:4][C:5](=[O:8])[NH:6][CH:7]=1.[C@@H:10]1(N2C3N=C(N)NC(=O)C=3N=C2)[O:18][C@H:15]([CH2:16][OH:17])[C@@H:13]([OH:14])[C@H:11]1[OH:12]>>[CH3:1][C:2]1[C:3](=[O:9])[NH:4][C:5](=[O:8])[N:6]([CH:7]=1)[C@@H:10]1[O:18][C@H:15]([CH2:16][OH:17])[C@@H:13]([OH:14])[C@H:11]1[OH:12]

参加物

3

反応物 2

HCID 1135ThymineHCID 135398635Guanosine

生成物 1

HCID 4454085-Methyluridine

実験手順

A culture broth of Flavobacterium rhenanum FERM BP-1862 was prepared according to Example 1 of JP 2-23882. 5-Methyluracil and guanosine as substrates were added without removing the culture medium ingredients of the strain culture solution, and were reacted while keeping the temperature at 60° C. in the same manner as in Example 1 of the present invention. The reaction solution was cooled, filtered, decolored with activated carbon, refiltered and concentrated in vacuo. The concentrate was recooled to 5° C. The particle diameter of the obtained 5-methyluridine crystals was from 20 to 30 μm. The separation phenomenon of 5-methyluridine crystals from other crystals of nucleic acids formed as impurities was not observed. Further, the centrifugal separation took much time.

後処理

  1. customA culture broth of Flavobacterium rhenanum FERM BP-1862 was prepared
  2. customwithout removing the culture medium ingredients of the strain culture solution
  3. customwere reacted
  4. customat 60° C.
  5. temperatureThe reaction solution was cooled
  6. filtrationfiltered
  7. concentrationconcentrated in vacuo
  8. customwas recooled to 5° C

出典と根拠

ORDレコード
ord-2fa94e55415d4fbc9f02525159d50768
出典データセット
uspto-grants-1996_08
特許番号
US05547857

関連ノート

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