反応式
CNC1(C2C=CC=CC=2Cl)C(=O)CCCC1.CC1C=CC=C(C)C=1NC1SCCCN=1.CC(C1C=CC2SC3C=CC=CC=3N(CCCN(C)C)C=2C=1)=O.[CH:55]1[C:60]([OH:61])=[C:59]([OH:62])[CH:58]=[C:57](O)[C:56]=1[CH2:64][CH2:65][NH2:66].O=C1O[C@H]([C@H](CO)O)C(O)=C1O>>[NH2:66][CH2:65][CH2:64][C:56]1[CH:57]=[CH:58][C:59]([OH:62])=[C:60]([OH:61])[CH:55]=1参加物
6
実験手順
Rats were anesthetized with a 1.0 ml/kg intramuscular injection of a mixture of ketamine (33 mg/ml), xylazine (1.7 mg/ml), and acepromazine (10 mg/ml) and positioned in a Kopf stereotaxic instrument. A total of 12 μg of 6-OHDA (6 μl volume at a concentration of 2 μg/μl dissolved in 0.9% saline containing 0.2 μg/μl ascorbic acid) were infused at a rate of 1.0 μl/minute and allowed to diffuse for 5 minutes before the infusion cannula was slowly retracted. Infusion coordinates were 4.2 mm posterior to bregma, 1.0 mm lateral from midline, and 7.4 mm ventral to dura.
出典と根拠
- ORDレコード
- ord-904a45114da049249fe459660907dbc1
- 出典データセット
- uspto-grants-1996_08
- 特許番号
- US05550050
関連ノート
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