Tyramine → Biotin tyramine

HRID 1080603

反応式

HRID 1080603 の反応式
C1S[C@@H](CCCCC(NCCCC[C@H](N)C(O)=O)=O)[C@H]2NC(N[C@@H]12)=O.[NH2:26][CH2:27][CH2:28][C:29]1[CH:34]=[CH:33][C:32]([OH:35])=[CH:31][CH:30]=1.C1C(=O)N([O:43][C:44]([CH2:46][CH2:47][CH2:48][CH2:49][CH:50]2[S:54][CH2:53][CH:52]3[NH:55][C:56]([NH:58][CH:51]23)=[O:57])=[O:45])C(=O)C1>>[OH:45][C:44]([CH2:46][CH2:47][CH2:48][CH2:49][C@H:50]1[C@@H:51]2[C@@H:52]([NH:55][C:56]([NH:58]2)=[O:57])[CH2:53][S:54]1)=[O:43].[NH2:26][CH2:27][CH2:28][C:29]1[CH:34]=[CH:33][C:32]([OH:35])=[CH:31][CH:30]=1

参加物

4

反応物 3

HCID 5610TyramineHCID 83814BiocytinHCID 434213NHS-Biotin

生成物 1

HCID 86746652Biotin tyramine

実験手順

Para-hydroxyphenylpropionyl biocytin (HPPB) was prepared by mixing a solution of p-hydroxyphenylpropionic acid-N-hydroxysuccinimide ester (50 mg [0.2 mMol]/2 ml dimethyl sulfoxide) with biocytin (70.75 mg [0.2 mMol]/2 ml 0.1M NaHCO3) overnight at room temperature (RT). Biotin-tyramine (BT) was prepared by mixing a solution of tyramine (40 mg [0.3 mMol]/1 ml dimethyl sulfoxide) with biotin-N-hydroxysuccinimide ester (100 mg [0.3 mMol]/1 ml dimethyl sulfoxide) overnight at RT. The solutions of HPPB and BT were used as is. The calculated concentrations were 26 mg/ml for HPPB and 55 mg/ml for BT.

後処理

  1. concentrationThe calculated concentrations

出典と根拠

ORDレコード
ord-eb10b5356f1040039f7afd213056b076
出典データセット
uspto-grants-1996_12
特許番号
US05583001

関連ノート

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