Methanol → Genipin

HRID 1089629ノート

反応式

HRID 1089629 の反応式
[CH3:1][O:2][C:3]([C:5]1[C@H:10]2[CH2:11][CH:12]=[C:13]([CH2:14][OH:15])[C@H:9]2[C@H:8]([O:16][C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[O:7][CH:6]=1)=[O:4].C(Cl)(Cl)Cl.CO.S(=O)(=O)(O)O>O>[CH3:1][O:2][C:3]([C:5]1[C@H:10]2[CH2:11][CH:12]=[C:13]([CH2:14][OH:15])[C@H:9]2[C@H:8]([OH:16])[O:7][CH:6]=1)=[O:4]

参加物

6

反応物 4

HCID 887MethanolHCID 1118Sulfuric AcidHCID 6212ChloroformHCID 107848Geniposide

生成物 1

HCID 442424Genipin収率 67.3%

試薬・触媒・溶媒 1

HCID 962Water溶媒

実験手順

To a solution of geniposide (6.5 g) dissolved in distilled water (65 ml), was added β-glucosidase (No.G-0395, β-D-glucoside glucohydrase EC 3.2.1.21) (316.55 mg, 1677.72 units) the mixture was incubated at 37° C. for 4.5 hours. The progress of the reaction was monitored by TLC (chloroform:methanol=8:2, visualized with 50%-sulfuric acid). After the reaction was complete, the reaction solution was extracted several times with ethyl acetate, and subsequently the ethyl acetate layer was concentrated to give genipin as white needles (2.55 g, yield 67.3%).

後処理

  1. extractionthe reaction solution was extracted several times with ethyl acetate
  2. concentrationsubsequently the ethyl acetate layer was concentrated

出典と根拠

ORDレコード
ord-dfa9eb9e0d0049099466fed33b18ae6f
出典データセット
uspto-grants-1992_01
特許番号
US05078750

反応ノート

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