Dicyclohexylcarbodiimide → HCID 178513115、化合物

HRID 1168668

反応式

HRID 1168668 の反応式
[NH:1]([CH:5]([S:11][S:12][CH2:13][C@H:14]([NH2:18])[C:15]([OH:17])=[O:16])[C@H:6]([NH2:10])[C:7]([OH:9])=[O:8])[C:2]([NH2:4])=[NH:3].C1(N=C=NC2CCCCC2)CCCCC1.ON1C(=O)CCC1=O.[NH2:42][CH2:43][CH2:44][CH2:45][N:46]1[CH2:51][CH2:50][N:49]([CH2:52][CH2:53][CH2:54][NH2:55])[CH2:48][CH2:47]1>O.O1CCOCC1>[NH2:55][CH2:54][CH2:53][CH2:52][N:49]1[CH2:48][CH2:47][N:46]([CH2:45][CH2:44][CH2:43][NH2:42])[CH2:51][CH2:50]1.[NH:1]([CH:5]([S:11][S:12][CH2:13][C@H:14]([NH2:18])[C:15]([OH:17])=[O:16])[C@H:6]([NH2:10])[C:7]([OH:9])=[O:8])[C:2]([NH2:4])=[NH:3]

参加物

7

反応物 4

HCID 10868DicyclohexylcarbodiimideHCID 80170N-HydroxysuccinimideHCID 816291,4-Bis(3-aminopropyl)piperazineHCID 178418808HCID 178418808、化合物

生成物 1

HCID 178513115HCID 178513115、化合物収率 5%

試薬・触媒・溶媒 2

HCID 962Water溶媒HCID 312751,4-Dioxane溶媒

実験手順

To a solution of guanidino-L-cystine (64 mg, 0.2 mmol) in water (10 ml) was slowly added N,N′-dicyclohexylcarbodiimide (82 mg, 0.4 mmol) and N-hyroxysuccinimide (46 mg, 0.4 mmol) in dioxane (5 ml). After 16 hr, the solution was filtered through a cotton plug and 1,4-bis(3-aminopropyl)piperazine (40 μL, 0.2 mmol) was added. The reaction was allowed to stir at room temperature for 16 h and then the aqueous solution was dialyzed in a 15,000 MW cutoff tubing against water (2×2 l) for 24 h. The solution was then removed from dialysis tubing, filtered through 5 μM nylon syringe filter and then dried by lyophilization to yield 5 mg of polymer.

後処理

  1. additionwas added
  2. customthe aqueous solution was dialyzed in a 15,000 MW cutoff
  3. waittubing against water (2×2 l) for 24 h
  4. customThe solution was then removed from dialysis tubing
  5. filtrationfiltered through 5 μM nylon syringe
  6. filtrationfilter
  7. customdried by lyophilization

出典と根拠

ORDレコード
ord-6dfa9c60e5e04947a2a28119efd5511b
出典データセット
uspto-grants-2003_09
特許番号
US06627616B2

関連ノート

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