Sodium Chloride → Dopamine

HRID 1196914

反応式

HRID 1196914 の反応式
C1C2(N(C3C=CC=CC=3)CNC2=O)CCN(CCC[C:21]([C:23]2[CH:28]=[CH:27][C:26](F)=CC=2)=[O:22])C1.C(O)[C:31](N)([CH2:34][OH:35])[CH2:32]O.Cl.[Na+].[Cl-].CC([C@]1(O)C[C@H]2[N:48](C[C@H]3C4C2=CC=CC=4CCC2C=CC=CC3=2)CC1)(C)C>>[NH2:48][CH2:26][CH2:27][C:28]1[CH:32]=[CH:31][C:34]([OH:35])=[C:21]([OH:22])[CH:23]=1

参加物

5

反応物 4

HCID 5234Sodium ChlorideHCID 5265SpiperoneHCID 37461ButaclamolHCID 93573Tromethamine hydrochloride

生成物 1

HCID 681Dopamine

実験手順

0.5 mL aliquots of the membrane preparation were incubated with unlabeled drugs, and 0.15 nM [3H]spiperone in a final volume of 1 mL containing 50 mM Tris HCl, 120 mM NaCl and 1 mM MgC12 (pH 7.7). Nonspecific binding was measured in the presence of 100 nM (+)-butaclamol. After 15 minutes of incubation at 37° C., samples were filtered rapidly through Whatman GF/C glass filters under negative pressure, and washed three times with ice-cold binding buffer (5 mL).

後処理

  1. custom0.5 mL aliquots of the membrane preparation
  2. filtrationsamples were filtered rapidly through Whatman GF/C glass filters under negative pressure
  3. washwashed three times with ice-cold binding buffer (5 mL)

出典と根拠

ORDレコード
ord-4da49f3c9dc14a6fb1b8f9a51fe753b2
出典データセット
uspto-grants-1992_05
特許番号
US05116846

関連ノート

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