Dicyclohexylcarbodiimide → Nitrilotriacetic Acid
反応式
C1(N=C=NC2CCCCC2)CCCCC1.ON1C(=O)CCC1=O.[C:24]([CH2:27][N:28]([CH2:38][C:39]([OH:41])=[O:40])[C@H:29]([C:35]([OH:37])=[O:36])CCCCN)([OH:26])=[O:25]>>[N:28]([CH2:27][C:24]([OH:26])=[O:25])([CH2:29][C:35]([OH:37])=[O:36])[CH2:38][C:39]([OH:41])=[O:40]参加物
4
実験手順
10 mg of carboxy-modified cores were washed twice with 1 ml of acetonitrile (MeCN) and then taken up in 1 ml of MeCN. To this were added 10 μmol of dicyclohexylcarbodiimide and 10 μmol of N-hydroxysuccinimide. This was followed by shaking at room temperature for two hours. Washing was then carried out once with 1 ml of cyclohexane and once with 1 ml of MeCN. The particles were then taken up in 1 ml of MeCN. 4 μmol of N,N-biscarboxymethyl-L-lysine were added thereto and shaken at room temperature for three hours. This was followed by washing once with 1 ml of acetonitrile and twice with 1 ml of 10 mM phosphate buffer (pH 7.0).
後処理
- washwere washed twice with 1 ml of acetonitrile (MeCN)
- washWashing
- stirringshaken at room temperature for three hours
- washby washing once with 1 ml of acetonitrile and twice with 1 ml of 10 mM phosphate buffer (pH 7.0)
出典と根拠
- ORDレコード
- ord-abf830deaa8342fbbb8d6499ef494193
- 出典データセット
- uspto-grants-2007_04
- 特許番号
- US07202472B2
反応ノート
読み込み中…