Diisopropylethylamine → H-Gly-phe-leu-OH

HRID 1271011

反応式

HRID 1271011 の反応式
[NH:1](C(OC(C)(C)C)=O)[CH2:2][C:3]([NH:5][C@H:6]([C:14]([NH:16][C@H:17]([C:22]([OH:24])=[O:23])[CH2:18][CH:19]([CH3:21])[CH3:20])=[O:15])[CH2:7][C:8]1[CH:13]=[CH:12][CH:11]=[CH:10][CH:9]=1)=[O:4].N1(O[P+](N(C)C)(N(C)C)N(C)C)C2C=CC=CC=2N=N1.CCN(C(C)C)C(C)C>CN(C=O)C>[NH2:1][CH2:2][C:3]([NH:5][C@H:6]([C:14]([NH:16][C@H:17]([C:22]([OH:24])=[O:23])[CH2:18][CH:19]([CH3:20])[CH3:21])=[O:15])[CH2:7][C:8]1[CH:13]=[CH:12][CH:11]=[CH:10][CH:9]=1)=[O:4]

参加物

5

反応物 3

HCID 81531DiisopropylethylamineHCID 124335Benzotriazol-1-yloxy-tris(dimethylamino)phosphoniumHCID 13943016N-(tert-Butoxycarbonyl)glycyl-L-phenylalanyl-L-leucine

生成物 1

HCID 7016074H-Gly-phe-leu-OH

試薬・触媒・溶媒 1

HCID 6228Dimethylformamide溶媒

実験手順

Boc-Gly-L-Phe-L-Leu-OH (44 mg, 0.1 mmol), benzotriazol-1-yloxytris(dimethylamino)phosphonium hexaflurophsophate (14 mg, 0.104 mmol) are dissolved in dry DMF (1 mL). DIEA (20 μL, 0.115 mmol) is added and about 0.5-1.0 mL of this solution is transferred to a test tube containing amino derivatized ELAMS™. The tube is sealed, vortexed for about 3.5-4 hours and the ELAMS™ pelleted and washed with DMF (3×) and methylene chloride (2×). The ELAMS™ are then deprotected with a solution of 50% triluoroacetic acid (TFA) is methylene chloride for 30 min., washed with methylene chloride (2×), ethanol (2×) and methylene chloride (2×), and dried at 55° C. for about 1 hour. The identifier tag from each ELAMS™, is detected and recorded.

後処理

  1. additioncontaining amino derivatized ELAMS™
  2. customThe tube is sealed
  3. washwashed with DMF (3×) and methylene chloride (2×)
  4. customfor 30 min.
  5. washwashed with methylene chloride (2×), ethanol (2×) and methylene chloride (2×)
  6. customdried at 55° C. for about 1 hour

出典と根拠

ORDレコード
ord-c655788b2c684be79c785ee2ed206f7a
出典データセット
uspto-grants-2007_04
特許番号
USRE039571E1

関連ノート

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