C(OC([N:8]1[CH2:13][CH2:12][N:11](C(OC(C)(C)C)=O)[CH2:10][C@@H:9]1[CH2:21][C@@H:22]([OH:24])[CH3:23])=O)(C)(C)C.C(OC(N1CCN(C(OC(C)(C)C)=O)C[C@@H]1C[C@H](O)C)=O)(C)(C)C.FC(F)(F)C(O)=O>ClCCl>[OH:24][C@H:22]([CH3:23])[CH2:21][C@H:9]1[CH2:10][NH:11][CH2:12][CH2:13][NH:8]1To a solution of 2(S)-(2(S)-hydroxypropyl) piperazine-1,4-dicarboxylic acid di-tert-butyl ester and 2(S)-(2(R)-hydroxypropyl) piperazine-1,4-dicarboxylic acid di-tert-butyl ester (5.11 g, 14.8 mmol) in dichloromethane (100 mL) stirring at 0° C., add trifluoroacetic acid (25 mL). Warm the mixture to room temperature and stir. After 18 hours evaporate to a yellow oil. Dilute the mixture with methanol and apply to a 60 g SCX column. Wash with methanol and elute with 2N ammonia in methanol to obtain the title compound (mixture of diastereoisomers) as an oil (1.932 g, 90%): mass spectrum (APCI): m/z =145.1 (M+1).
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