Dimethyl Sulfoxide → (+)-Eriodictyol

HRID 1391405

反応式

HRID 1391405 の反応式
[CH3:1][C@@H:2]1[O:6][C@@H:5]([O:7][C@H:8]2[C@H:13](O)[C@@H:12]([OH:15])[C@H:11](NC(N)=N)[C@@H:10]([OH:20])[C@@H:9]2NC(N)=N)[C@H:4](O[C@@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@:3]1(O)C=O.C1[C@H](N)[C@@H:45]([O:48][C@H]2O[C@H](CN)[C@@H](O)[C@H](O)[C@H]2O)[C@H:44](O)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](N)[C@H]2O)[C@@H]1N.[CH3:74][CH:75](C(NCC(O)=O)=S)C(N)=N.CS(C)=[O:88]>>[CH:74]1[C:4]([C@H:5]2[O:7][C:8]3[CH:9]=[C:10]([OH:20])[CH:11]=[C:12]([OH:15])[C:13]=3[C:45](=[O:48])[CH2:44]2)=[CH:3][C:2]([OH:6])=[C:1]([OH:88])[CH:75]=1

参加物

5

反応物 4

HCID 679Dimethyl SulfoxideHCID 6032KanamycinHCID 3028447N-(2-Amidinothiopropionyl)glycineHCID 178484872HCID 178484872、化合物

生成物 1

HCID 440735(+)-Eriodictyol

実験手順

Batch fermentations were prepared to determine the overall production of flavonoid compounds using commercially available (2S)-eriodictyol. An overnight preinnoculums of recombinant E. coli BL21Star (Invitrogen) was prepared carrying vectors pCDF-F and pCOLA-DL in LB or M9 minimal media. Antibiotics were added according to manufacturer's instructions (50 ug/mL streptomycin and 30 ug/mL kanamycin). The next day, 4 mL of the preinnoculum was used to seed 76 mL LB or M9 minimal media cultures. The culture was grown in an incubator at 37° C. until OD600 reaches about 0.6. Gene expression was induced by 1 mM ITPG and the cultures were grown at 27° C. with horizontal shaking for 4 hours. (2S)-Eriodictyol dissolved in DMSO was added to a final concentration of 0.2 mM. The cultures were incubated at 27° C. for 72 hours.

後処理

  1. customBatch fermentations were prepared
  2. customAn overnight
  3. customcoli BL21Star (Invitrogen) was prepared
  4. additionAntibiotics were added
  5. customwas grown in an incubator at 37° C. until OD600 reaches about 0.6
  6. customwere grown at 27° C.
  7. additionwas added to a final concentration of 0.2 mM
  8. waitThe cultures were incubated at 27° C. for 72 hours

出典と根拠

ORDレコード
ord-1305d5b06ce7479491079af4649bb51f
出典データセット
uspto-grants-2008_03
特許番号
US07338791B2

関連ノート

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