Sodium Bicarbonate → Prostaglandin E2

HRID 1461333

反応式

HRID 1461333 の反応式
O=C1O[C@H]([C@H](CO)O)C(O)=C1O.C[C@@H]1[C@@H](O)[C@@H](C)[C@H](C)[O:50][C:48](=[O:49])[CH2:47][C@H:46](O)[CH2:45][C@H:44](O)[CH2:43][CH2:42][C@@H:41](O)[C@H:40]([OH:60])[CH2:39][C@H:38]([OH:61])[CH2:37][C@@:35]2(O)O[C@H:31]([C@H:32](C(O)=O)[C@@H:33]([OH:63])[CH2:34]2)[CH2:30][C@@H:29](O[C@@H]2O[C@H](C)[C@@H](O)[C@H](N)[C@@H]2O)[CH:28]=CC=CC=CC=CC=CC=CC=C1.C([O-])(O)=O.[Na+].CC1(C)S[C@@H]2[C@H](NC(CC3C=CC=CC=3)=O)C(=O)N2[C@H]1C([O-])=O.[K+].C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]2NC(N)=N)[C@H](O[C@@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@]1(O)C=O>C(=O)=O>[CH3:28][CH2:29][CH2:30][CH2:31][CH2:32][C@H:33]([OH:63])/[CH:34]=[CH:35]/[C@@H:37]1[C@@H:41]([CH2:42]/[CH:43]=[CH:44]\[CH2:45][CH2:46][CH2:47][C:48]([OH:50])=[O:49])[C:40](=[O:60])[CH2:39][C@H:38]1[OH:61]

参加物

7

反応物 5

HCID 516892Sodium BicarbonateHCID 5280965Amphotericin BHCID 23664709Penicillin G PotassiumHCID 54670067L-Ascorbic AcidHCID 178484872HCID 178484872、化合物

生成物 1

HCID 5280360Prostaglandin E2

試薬・触媒・溶媒 1

HCID 280Carbon Dioxide溶媒

実験手順

Synovial tissue obtained at surgery from patients with rheumatoid arthritis (RA) undergoing joint replacement was cut into pieces of 2 mm or less and incubated at approximately 100 mg/ml in serum-free minimal essential medium (MEM) containing 1 mg/ml bacterial collagenase (Sigma Chemical Co., St. Louis, Mo.) and 10 μg/ml testicular hyaluronidase (Sigma) at 37° C. with rocking. After 3-4 h, the suspension was centrifuged at 150 xg for 10 min. The cells were resuspended (105 cells/ml) in MEM containing 20% fetal calf serum (FCS) and plated in 100-mm diameter Petri dishes (10 ml per dish). The disaggregated cells were cultured overnight at 37° C. in 5% CO2, and the nonadherent cells were aspirated off by vigorous pipetting. Adherent synovial cells were maintained in culture with medium change every four days until they reached confluency, at which time they were subcultured after trypsinization and grown in maintenance medium (Eagle's MEM Supplemented with nonessential amino acids, ascorbic acid, amphotericin B (1 μg/ml), NaHCO3, penicillin (100 U/ml), and streptomycin (100 μ/ml), FCS (9%), and PBS).

後処理

  1. customSynovial tissue obtained at surgery from patients with rheumatoid arthritis (RA)
  2. additioncontaining 1 mg/ml bacterial collagenase (Sigma Chemical Co., St. Louis, Mo.) and 10 μg/ml testicular hyaluronidase (Sigma) at 37° C. with rocking
  3. waitthe suspension was centrifuged at 150 xg for 10 min
  4. additioncontaining 20% fetal calf serum (FCS)
  5. temperatureAdherent synovial cells were maintained in culture with medium change every four days until they

出典と根拠

ORDレコード
ord-a52560b08e02425a964d5b7996870095
出典データセット
uspto-grants-1991_12
特許番号
US05075288

関連ノート

読み込み中…