Dianisidine → 2-Naphthol

HRID 1576739

反応式

HRID 1576739 の反応式
COC1C=[C:7]([C:9]2[CH:14]=[CH:13][C:12](N)=[C:11]([O:16]C)[CH:10]=2)[CH:6]=[CH:5][C:4]=1N.[OH-].[Na+]>>[CH:10]1[C:9]2[C:14](=[CH:4][CH:5]=[CH:6][CH:7]=2)[CH:13]=[CH:12][C:11]=1[OH:16]

参加物

3

反応物 2

HCID 8411DianisidineHCID 14798Sodium Hydroxide

生成物 1

HCID 86632-Naphthol

実験手順

Silica particles were derivatized with o-dianisidine by Method No. 2. After drying, 1.0 gm of the carrier was diazotized with sodium nitrite. After diazotization the derivative was washed several times with borate (0.03M) buffered saline (0.15M) pH 8.0. The derivative was then reacted with 1.5 ml of T4 antisera (titer 1:10000) at 4°C. with stirring. The pH was maintained between 8.0 - 8.5 with 0.1N NaOH. After 30 minutes the pH stabilized at 8.2 and the reaction was stirred slowly at 4°C. overnight. After this time the composite gave a negative β-napthol test indicating no more remaining functional diazo groups. The preparation was then washed (4X) with phosphate-buffer (0.03M) saline (0.15M) containing 0.1% bovine serum albumin (PBS-BSA).

後処理

  1. customAfter drying
  2. washAfter diazotization the derivative was washed several times with borate (0.03M)
  3. customThe derivative was then reacted with 1.5 ml of T4 antisera (titer 1:10000) at 4°C.
  4. stirringthe reaction was stirred slowly at 4°C. overnight

出典と根拠

ORDレコード
ord-2178f3db996a407b8d5833f978e270e8
出典データセット
uspto-grants-1976_09
特許番号
US03983000

関連ノート

読み込み中…