HCID 15932584、化合物 → HCID 131717986、化合物

HRID 1588116ノート

反応式

HRID 1588116 の反応式
[C:1]([NH:4][C@@H:5]1[C@@H:10]([OH:11])[C@H:9]([OH:12])[C@@H:8]([CH2:13][OH:14])[O:7][C@H:6]1[O:15][C@@H:16]1[C@@H:31]([OH:32])[C@@H:30]([OH:33])[C@@H:29]([CH2:34][O:35][C@@H:36]2[O:43][C@H:42]([CH2:44][OH:45])[C@@H:40]([OH:41])[C@H:38]([OH:39])[C@H:37]2[NH:46][C:47](=[O:49])[CH3:48])[O:28][C@H:17]1[O:18][CH2:19][CH2:20][CH2:21][CH2:22][CH2:23][C:24]([O:26][CH3:27])=[O:25])(=[O:3])[CH3:2].C1C(=O)NC(=O)NC=1[C@@H]1O[C@H](COP(OP(O[C@H:73]2[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]2[OH:83])(O)=O)(O)=O)[C@@H](O)[C@H]1O>[As](C)(C)(=O)[O-].[Na+].[Cl-].[Mn+2].[Cl-]>[C@@H:17]1([O:12][C@@H:9]2[C@@H:8]([CH2:13][OH:14])[O:7][C@@H:6]([O:15][C@@H:16]3[C@@H:31]([OH:32])[C@@H:30]([OH:33])[C@@H:29]([CH2:34][O:35][C@@H:36]4[O:43][C@H:42]([CH2:44][OH:45])[C@@H:40]([O:41][C@@H:73]5[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]5[OH:83])[C@H:38]([OH:39])[C@H:37]4[NH:46][C:47](=[O:49])[CH3:48])[O:28][C@H:17]3[O:18][CH2:19][CH2:20][CH2:21][CH2:22][CH2:23][C:24]([O:26][CH3:27])=[O:25])[C@H:5]([NH:4][C:1](=[O:3])[CH3:2])[C@H:10]2[OH:11])[O:28][C@H:29]([CH2:34][OH:35])[C@H:30]([OH:33])[C@H:31]([OH:32])[C@H:16]1[OH:15]

参加物

5

反応物 2

HCID 15932584HCID 15932584、化合物HCID 54584734HCID 54584734、化合物

生成物 1

HCID 131717986HCID 131717986、化合物

試薬・触媒・溶媒 2

HCID 10313134Manganese(2+) dichloride触媒HCID 2724247Sodium Cacodylate溶媒

実験手順

A solution of compound 17 of Example 17 (72 mg), UDP-galactose, (190 mg), bovine galactosyltransferase (6 U) and bovine serum albumin (3 mg) in 30 mM sodium cacodylate buffer (7 mL, pH 7.0) containing manganese chloride (40 umol) was incubated at 37° C. for 22 h. The reaction mixture was diluted to 20 mL and purified as described in Example 21. Yield of the product was 95 mg. The structural identity of titled compound 23 was unambiguously assigned by 1H- and 13C-n.m.r (see Tables 4 and 5, respectively).

後処理

  1. additionThe reaction mixture was diluted to 20 mL
  2. custompurified

出典と根拠

ORDレコード
ord-9f681f83b22a4c5f8a86b0858d153d48
出典データセット
uspto-grants-1993_06
特許番号
US05220008

反応ノート

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