5-Aminofluorescein → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

反応式

N=C=N.[CH:4]1[C:9]([NH2:10])=[CH:8][C:7]2[C:11]([O:13][C:14]3([C:24]4[CH:25]=[CH:26][C:27]([OH:29])=[CH:28][C:23]=4[O:22][C:16]4[CH:17]=[C:18]([OH:21])[CH:19]=[CH:20][C:15]3=4)[C:6]=2[CH:5]=1)=[O:12]>>[CH:4]1[CH:9]=[CH:8][C:7]([C:11]([OH:13])=[O:12])=[C:6]([C:14]2[C:15]3[CH:20]=[CH:19][C:18]([OH:21])=[CH:17][C:16]=3[O:22][C:23]3[C:24]=2[CH:25]=[CH:26][C:27]([CH:28]=3)=[O:29])[CH:5]=1.[CH:4]1[C:9]([NH2:10])=[CH:8][C:7]2[C:11]([O:13][C:14]3([C:24]4[CH:25]=[CH:26][C:27]([OH:29])=[CH:28][C:23]=4[O:22][C:16]4[CH:17]=[C:18]([OH:21])[CH:19]=[CH:20][C:15]3=4)[C:6]=2[CH:5]=1)=[O:12]

参加物

反応物 2

HCID 768455-Aminofluorescein

HCID 160435Carbodiimide

生成物 2

HCID 33832-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

HCID 768455-Aminofluorescein

実験手順

Alternatively, the product from step 1 can be reacted in the presence of carbodiimide with fluoresceinamine (FAM) (Reaction C) to provide 5-[2',3'-dideoxyinosin-5'-yl)-succinamido]-fluorescein (ddI-5'-S:FAM). An additional alternative is to react the product from step 2 with 5-[N'-(2-aminoethyl)-thioureido]fluorescein (fluoresceinthiocarbamylethylenediamine) (FTED) (Reaction D) to provide the tracer 5-[2-(2',3'-dideoxyinosin-5'-yl-succinamido)-ethylthiocarbamyl -fluorescein (ddI-5.-S:FTED).

出典と根拠

ORDレコード: ord-89b67904a1474a93abc56a053506daea
出典データセット: uspto-grants-1993_06
DOI: 10.6084/m9.figshare.5104873.v1
特許番号: US05221611

反応ノート

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