Sodium Chloride → 2-Isobutyl-3-ketoglutarate diethyl ester

HRID 1594429

反応式

HRID 1594429 の反応式
P([O-])([O-])([O-])=O.[K+].[K+].[K+].[Na+].[Cl-].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[CH2:23]([O:25][C:26](=[O:41])[CH:27]([CH2:37][CH:38]([CH3:40])[CH3:39])[C:28](=[O:36])[C:29](=O)[C:30]([O:32][CH2:33][CH3:34])=[O:31])[CH3:24]>C(OCC)(=O)C.OCC(CO)O.CS(C)=O>[CH2:23]([O:25][C:26](=[O:41])[CH:27]([CH2:37][CH:38]([CH3:39])[CH3:40])[C:28](=[O:36])[CH2:29][C:30]([O:32][CH2:33][CH3:34])=[O:31])[CH3:24]

参加物

8

反応物 4

HCID 5234Sodium ChlorideHCID 62657Potassium Phosphate, TribasicHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2 回HCID 220229102-Isobutyl-3-ketoketoglutarate diethyl ester

生成物 1

HCID 99926592-Isobutyl-3-ketoglutarate diethyl ester

試薬・触媒・溶媒 3

HCID 679Dimethyl Sulfoxide溶媒HCID 753Glycerol溶媒HCID 8857Ethyl Acetate溶媒

実験手順

To 40 μL of 300 mM potassium phosphate buffer, pH=6.5, containing NaCl (100 mM), DMSO (3% v:v), glucose (200 mM), and glycerol (10% v:v), 40 mM of 2-isobutyl-3-ketoketoglutarate diethyl ester was added along with 100 mg of lyophilized KRED 1001 and 30 mg of glucose dehydrogenase. The reaction mixture was incubated for 48 hours at 37° C. Gas chromatographic analysis showed that the yield of hydroxy diester product was greater than 80%. The product was isolated by extraction of the reaction mixture with ethyl acetate and purification using silica gel chromatography. Isolated yield was in the range of 85–95%.

後処理

  1. customIsolated
  2. customyield

出典と根拠

ORDレコード
ord-0ef17882d33c428eb99d0d14024b2fc0
出典データセット
uspto-grants-2007_11
特許番号
US07301031B2

関連ノート

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