Diisopropylethylamine → (2S,4R)-1-(2-(2H-1,2,3-triazol-2-yl)acetyl)-4-(4-fluorobenzyl)-N-(4-(4-fluorophenoxy)phenyl)pyrrolidine-2-carboxamide

HRID 1630300ノート

反応式

HRID 1630300 の反応式
Cl.[F:2][C:3]1[CH:31]=[CH:30][C:6]([CH2:7][C@H:8]2[CH2:12][NH:11][C@H:10]([C:13]([NH:15][C:16]3[CH:21]=[CH:20][C:19]([O:22][C:23]4[CH:28]=[CH:27][C:26]([F:29])=[CH:25][CH:24]=4)=[CH:18][CH:17]=3)=[O:14])[CH2:9]2)=[CH:5][CH:4]=1.[N:32]1[N:33]([CH2:37][C:38](O)=[O:39])[N:34]=[CH:35][CH:36]=1.CN(C(ON1N=NC2C=CC=NC1=2)=[N+](C)C)C.F[P-](F)(F)(F)(F)F.CCN(C(C)C)C(C)C>CN(C=O)C>[N:32]1[N:33]([CH2:37][C:38]([N:11]2[CH2:12][C@H:8]([CH2:7][C:6]3[CH:5]=[CH:4][C:3]([F:2])=[CH:31][CH:30]=3)[CH2:9][C@H:10]2[C:13]([NH:15][C:16]2[CH:21]=[CH:20][C:19]([O:22][C:23]3[CH:28]=[CH:27][C:26]([F:29])=[CH:25][CH:24]=3)=[CH:18][CH:17]=2)=[O:14])=[O:39])[N:34]=[CH:35][CH:36]=1

参加物

6

反応物 4

HCID 81531DiisopropylethylamineHCID 9886157Methanaminium, N-((dimethylamino)(3H-1,2,3-triazolo(4,5-b)pyridin-3-yloxy)methylene)-N-methyl-, hexafluorophosphate(1-) (1:1)HCID 234341642-(2H-1,2,3-triazol-2-yl)acetic acidHCID 67010493(2S,4R)-4-(4-fluorobenzyl)-N-(4-(4-fluorophenoxy)phenyl)pyrrolidine-2-carboxamide hydrochloride

生成物 1

HCID 45377016(2S,4R)-1-(2-(2H-1,2,3-triazol-2-yl)acetyl)-4-(4-fluorobenzyl)-N-(4-(4-fluorophenoxy)phenyl)pyrrolidine-2-carboxamide収率 89.3%

試薬・触媒・溶媒 1

HCID 6228Dimethylformamide溶媒

実験手順

A round bottom flask was charged with (2S,4R)-4-(4-fluorobenzyl)-N-(4-(4-fluorophenoxy)phenyl)pyrrolidine-2-carboxamide hydrochloride (150 mg, 0.37 mmole, 1 eq), prepared as in Reference 6, 2H-1,2,3-triazole-2-ylacetic acid (51 mg, 0.40 mmole, 1.1 eq), HATU (152 mg, 0.40 mmole, 1.1 eq) and DMF (1 mL). DIEA (195 μL, 1.11 mmole, 3 eq) was added and the mixture stirred at ambient temperature for 18 hours. The reaction mixture was partitioned between ethyl acetate and deionized water. The aqueous layer was separated and extracted twice with ethyl acetate. The combined organics were washed with 5% lithium chloride (3×5 mL), 1N sodium bicarbonate (2×5 mL) and then deionized water (2×5 mL) and concentrated in vacuo. Product was purified from the residue via flash chromatography (silica, 5% MeOH, 95% EtOAc). Clean fractions were combined and concentrated in vacuo to afford Compound 50, (2S,4R)-1-(2-(2H-1,2,3-triazol-2-yl)acetyl)-4-(4-fluorobenzyl)-N-(4-(4-fluorophenoxy)phenyl)pyrrolidine-2-carboxamide (171 mg, 89% yield), as a white solid. Major isomer: 1H-NMR (400 MHz, DMSO-D6): σ 9.97 (s, 1H), 7.80 (s, 2H), 7.58-7.51 (m, 2H), 7.34-7.07 (m, 6H), 7.04-6.90 (m, 4H), 5.48 (q, 2H), 4.53-4.46 (m, 1H), 3.85-3.76 (m, 1H), 2.77-2.61 (m, 3H), 2.03-1.86 (m, 2H). MS (EI) for C28H25F2N5O3. found 517.9 (MH+).

後処理

  1. customThe reaction mixture was partitioned between ethyl acetate
  2. customThe aqueous layer was separated
  3. extractionextracted twice with ethyl acetate
  4. washThe combined organics were washed with 5% lithium chloride (3×5 mL), 1N sodium bicarbonate (2×5 mL)
  5. concentrationconcentrated in vacuo
  6. customProduct was purified from the residue via flash chromatography (silica, 5% MeOH, 95% EtOAc)
  7. concentrationconcentrated in vacuo

出典と根拠

ORDレコード
ord-6ab314c0324f4baba1a4ff58d791c264
出典データセット
uspto-grants-2014_07
特許番号
US08791102B2

反応ノート

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