Diisopropylethylamine → 6-Azido-2-fluoro-9-(2,3,5-tri-O-benzyl-beta-D-arabinofuranosyl)purine

HRID 1646666ノート

反応式

HRID 1646666 の反応式
[CH2:1]([O:8][C@H:9]1[C@H:25]([O:26][CH2:27][C:28]2[CH:33]=[CH:32][CH:31]=[CH:30][CH:29]=2)[C@@H:24]([CH2:34][O:35][CH2:36][C:37]2[CH:42]=[CH:41][CH:40]=[CH:39][CH:38]=2)[O:23][CH:10]1OC(=O)C1C=CC([N+]([O-])=O)=CC=1)[C:2]1[CH:7]=[CH:6][CH:5]=[CH:4][CH:3]=1.C(N(CC)C(C)C)(C)C.[N:52]([C:55]1[N:63]=[C:62]([F:64])[N:61]=[C:60]2[C:56]=1[NH:57][CH:58]=[N:59]2)=[N+:53]=[N-:54]>ClCCCl.ClCCl>[N:52]([C:55]1[N:63]=[C:62]([F:64])[N:61]=[C:60]2[C:56]=1[N:57]=[CH:58][N:59]2[C@@H:10]1[O:23][C@H:24]([CH2:34][O:35][CH2:36][C:37]2[CH:42]=[CH:41][CH:40]=[CH:39][CH:38]=2)[C@@H:25]([O:26][CH2:27][C:28]2[CH:33]=[CH:32][CH:31]=[CH:30][CH:29]=2)[C@@H:9]1[O:8][CH2:1][C:2]1[CH:7]=[CH:6][CH:5]=[CH:4][CH:3]=1)=[N+:53]=[N-:54]

参加物

6

反応物 3

HCID 81531Diisopropylethylamine2 回HCID 970762,3,5-Tri-O-benzyl-1-O-(4-nitrobenzoyl)-D-arabinofuranoseHCID 198012676-azido-2-fluoro-7H-purine

生成物 1

HCID 544897616-Azido-2-fluoro-9-(2,3,5-tri-O-benzyl-beta-D-arabinofuranosyl)purine収率 24.6%

試薬・触媒・溶媒 2

HCID 111,2-Dichloroethane溶媒HCID 6344Dichloromethane溶媒

実験手順

A solution of 2,3,5-tri-O-benzyl-1-α-D-arabinofuranosylchloride (freshly prepared from 1.9 g of 2,3,5-tri-O-benzyl-1-O-p-nitrobenzoyl-D-arabinofuranose), N,N-diisopropylethylamine (0.58 mL) and 6-azido-2-fluoropurine (0.50 g) in 10 mL of anhydrous 1,2-dichloroethane was heated at reflux overnight. Additional N,N-diisopropylethylamine (0.26 mL) was added and heating was continued for an additional 24 hours. The mixture was cooled to ambient temperature and diluted with dichloromethane (60 mL) and washed sequentially with 10% aqueous sodium hydroxide (NaOH), water, 1N phosphoric acid (H3PO4), saturated NaCl and then dried over MgSO4 along with decolorizing carbon. The dried solution was filtered through celite and concentrated in vacuo. The residue was purified by silica gel chromatography with gradient elution from 3:1 Hexane:ethyl acetate to 100% ethyl acetate to obtain 0.40 g of the title compound as a colorless oil; IR (neat) 3031, 2922, 2867, 2126, 1608 cm-1 ; 1H NMR (DMSO-d6, 360 MHz) δ3.65-3.75 (m, 2H), 4.16 (q, J=5 Hz, 1H), 4.22 (d, J=11.8 Hz), 4.42 (t, J=5.5 Hz, 1H), 4.47 (d, J=11.8 Hz, 1H), 4.51 (s, 2H), 4.58 (t, J=5.5 Hz, 1H), 4.62 (d, J=12.0 Hz, 1H), 4.68 (d, J=12.0 Hz, 1H), 6.41 (d, J=5.5 Hz, 1H), 6.9-6.95 (m, 2H) 7.1-7.2 (m, 3H), 7.25-7.4 (m, 10H), 8.48 (s, 1H); UV (MeOH) λmax, 286 nm; MS (EI+, m/z) 582 (M+H)+.

後処理

  1. temperaturewas heated
  2. temperatureat reflux overnight
  3. temperatureheating
  4. washwashed sequentially with 10% aqueous sodium hydroxide (NaOH), water, 1N phosphoric acid (H3PO4), saturated NaCl
  5. dry with materialdried over MgSO4 along with decolorizing carbon
  6. filtrationThe dried solution was filtered through celite and
  7. concentrationconcentrated in vacuo
  8. customThe residue was purified by silica gel chromatography with gradient elution from 3:1 Hexane

出典と根拠

ORDレコード
ord-c2e760113de6479bb1270021495b60a8
出典データセット
uspto-grants-1993_01
特許番号
US05180824

反応ノート

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