HCID 15932586、化合物 → HCID 54372859、化合物

HRID 1656865ノート

反応式

HRID 1656865 の反応式
[C:1]([NH:4][C@@H:5]1[C@@H:10]([OH:11])[C@H:9]([OH:12])[C@@H:8]([CH2:13][OH:14])[O:7][C@H:6]1[O:15][C@H:16]1[C@@H:31]([OH:32])[C@@H:30]([CH2:33][O:34][C@@H:35]2[O:42][C@H:41]([CH2:43][OH:44])[C@@H:39]([OH:40])[C@H:37]([OH:38])[C@H:36]2[NH:45][C:46](=[O:48])[CH3:47])[O:29][C@@H:18]([O:19][CH2:20][CH2:21][CH2:22][CH2:23][CH2:24][C:25]([O:27][CH3:28])=[O:26])[C@@H:17]1[OH:49])(=[O:3])[CH3:2].C1C(=O)NC(=O)NC=1[C@@H]1O[C@H](COP(OP(O[C@H:73]2[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]2[OH:83])(O)=O)(O)=O)[C@@H](O)[C@H]1O>[As](C)(C)(=O)[O-].[Na+].[Cl-].[Mn+2].[Cl-]>[C@@H:18]1([O:12][C@@H:9]2[C@@H:8]([CH2:13][OH:14])[O:7][C@@H:6]([O:15][C@H:16]3[C@@H:31]([OH:32])[C@@H:30]([CH2:33][O:34][C@@H:35]4[O:42][C@H:41]([CH2:43][OH:44])[C@@H:39]([O:40][C@@H:73]5[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]5[OH:83])[C@H:37]([OH:38])[C@H:36]4[NH:45][C:46](=[O:48])[CH3:47])[O:29][C@@H:18]([O:19][CH2:20][CH2:21][CH2:22][CH2:23][CH2:24][C:25]([O:27][CH3:28])=[O:26])[C@@H:17]3[OH:49])[C@H:5]([NH:4][C:1](=[O:3])[CH3:2])[C@H:10]2[OH:11])[O:29][C@H:30]([CH2:33][OH:34])[C@H:31]([OH:32])[C@H:16]([OH:15])[C@H:17]1[OH:49]

参加物

5

反応物 2

HCID 15932586HCID 15932586、化合物HCID 54584734HCID 54584734、化合物

生成物 1

HCID 54372859HCID 54372859、化合物

試薬・触媒・溶媒 2

HCID 10313134Manganese(2+) dichloride触媒HCID 2724247Sodium Cacodylate溶媒

実験手順

A solution of compound 19 of Example 19 (85 mg), UDP-galactose, (190 mg), bovine galactosyltransferase (6.25 U) and bovine serum albumin (3 mg) in 30 mM sodium cacodylate buffer (7 mL, pH 7.0) containing manganese chloride (40 umol) was incubated at 37° C. for 22 h. The reaction mixture was diluted to 20 mL and purified as described in Example 21. Yield of the product was 114 mg. The structural identity of titled compound 25 was unambiguously assigned by 1H-- and 13C-n.m.r (see Tables 4 and 5, respectively).

後処理

  1. additionThe reaction mixture was diluted to 20 mL
  2. custompurified

出典と根拠

ORDレコード
ord-662edec51c6a4ed5bc6e868b0dc61d31
出典データセット
uspto-grants-1993_10
特許番号
US05254676

反応ノート

読み込み中…