HCID 15932587、化合物 → HCID 54227580、化合物

HRID 1656866ノート

反応式

HRID 1656866 の反応式
[C:1]([NH:4][C@@H:5]1[C@@H:10]([OH:11])[C@H:9]([OH:12])[C@@H:8]([CH2:13][OH:14])[O:7][C@H:6]1[O:15][C@H:16]1[C@@H:31]([CH2:32][O:33][C@@H:34]2[O:41][C@H:40]([CH2:42][OH:43])[C@@H:38]([OH:39])[C@H:36]([OH:37])[C@H:35]2[NH:44][C:45](=[O:47])[CH3:46])[O:30][C@@H:19]([O:20][CH2:21][CH2:22][CH2:23][CH2:24][CH2:25][C:26]([O:28][CH3:29])=[O:27])[C@H:18]([OH:48])[C@H:17]1[OH:49])(=[O:3])[CH3:2].C1C(=O)NC(=O)NC=1[C@@H]1O[C@H](COP(OP(O[C@H:73]2[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]2[OH:83])(O)=O)(O)=O)[C@@H](O)[C@H]1O>[As](C)(C)(=O)[O-].[Na+].[Cl-].[Mn+2].[Cl-]>[C@@H:19]1([O:12][C@@H:9]2[C@@H:8]([CH2:13][OH:14])[O:7][C@@H:6]([O:15][C@H:16]3[C@@H:31]([CH2:32][O:33][C@@H:34]4[O:41][C@H:40]([CH2:42][OH:43])[C@@H:38]([O:39][C@@H:73]5[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]5[OH:83])[C@H:36]([OH:37])[C@H:35]4[NH:44][C:45](=[O:47])[CH3:46])[O:30][C@@H:19]([O:20][CH2:21][CH2:22][CH2:23][CH2:24][CH2:25][C:26]([O:28][CH3:29])=[O:27])[C@H:18]([OH:48])[C@H:17]3[OH:49])[C@H:5]([NH:4][C:1](=[O:3])[CH3:2])[C@H:10]2[OH:11])[O:30][C@H:31]([CH2:32][OH:33])[C@H:16]([OH:15])[C@H:17]([OH:49])[C@H:18]1[OH:48]

参加物

5

反応物 2

HCID 15932587HCID 15932587、化合物HCID 54584734HCID 54584734、化合物

生成物 1

HCID 54227580HCID 54227580、化合物

試薬・触媒・溶媒 2

HCID 10313134Manganese(2+) dichloride触媒HCID 2724247Sodium Cacodylate溶媒

実験手順

A solution of compound 20 of Example 20 (72 mg), UDP-galactose, (190 mg), bovine galactosyltransferase (6.25 U) and bovine serum albumin (3 mg) in 30 mM sodium cacodylate buffer (7 mL, pH 7.0) containing manganese chloride (40 umol) was incubated at 37° C. for 22 h. The reaction mixture was diluted to 20 mL and purified as described in Example 21. Yield of the product was 98 mg. The structural identity of titled compound 26 was unambiguously assigned by 1H-- and 13C-n.m.r (see Tables 4 and 5, respectively).

後処理

  1. additionThe reaction mixture was diluted to 20 mL
  2. custompurified

出典と根拠

ORDレコード
ord-58e7e751f49346a7a40069fc1370da35
出典データセット
uspto-grants-1993_10
特許番号
US05254676

反応ノート

読み込み中…