Adiponitrile → Ammonium adipate

HRID 1682416ノート

反応式

HRID 1682416 の反応式
C(#N)CCCCC#[N:7].C([CH2:11][CH2:12][CH2:13][CH2:14][C:15](N)=[O:16])#[N:10].C(N)(=O)CCCC[C:23]([NH2:25])=[O:24].C(CCCCC([O-])=O)#N.C([O-])(=O)CCCCC(N)=O.[C:47]([O-:56])(=[O:55])[CH2:48][CH2:49][CH2:50][CH2:51][C:52]([O-:54])=[O:53]>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[C:23]([O:24][C:15](=[O:16])[CH2:14][CH2:13][CH2:12][CH3:11])#[N:25].[C:47]([O-:56])(=[O:55])[CH2:48][CH2:49][CH2:50][CH2:51][C:52]([O-:54])=[O:53].[NH4+:7].[NH4+:10]

参加物

9

反応物 6

HCID 8128AdiponitrileHCID 12364HexanediamideHCID 200164Adipate(2-)HCID 117685765-CyanovaleramideHCID 226233665-CyanovalerateHCID 25795652Adipamate

生成物 2

HCID 167786Ammonium adipateHCID 22074301Cyanovalerate

試薬・触媒・溶媒 1

HCID 62657Potassium Phosphate, Tribasic溶媒

実験手順

A cellular residue containing 13.1 mg of dry weight of cells was suspended in 2 ml of a 52.3 mM solution of adiponitrile in 50 mM potassium phosphate buffer, pH 7. The reaction was carried out at 25° C., with shaking, and the kinetics were followed by sampling. 5-Cyanovaleramide, adipamide, 5-cyanovalerate, adipamate and adipate were determined on each sample by high performance liquid chromatography (HPLC). The results are collated in FIG. 1, which shows the curves of the yield (on the ordinate) of cyanovalerate (curve a) and ammonium adipate (curve b). The respective rates of formation of cyanovalerate and adipate were greater than 0.45 and equal to 0.15 U/mg of dry weight of cells (1 U is equal to 1 μmol of product formed per minute).

後処理

  1. additionA cellular residue containing 13.1 mg of dry weight of cells
  2. customwas carried out at 25° C.
  3. aliquotby sampling

出典と根拠

ORDレコード
ord-489f94c6b02649feb81c5fc5b08667fe
出典データセット
uspto-grants-1997_06
特許番号
US05635391

反応ノート

読み込み中…