L-Proline → cis-3-Hydroxy-L-proline

HRID 1737168

反応式

HRID 1737168 の反応式
[NH:1]1[CH2:8][CH2:7][CH2:6][C@H:2]1[C:3]([OH:5])=[O:4].[O:9]=C(CCC([O-])=O)C([O-])=O>>[OH:9][C@@H:6]1[CH2:7][CH2:8][NH:1][C@@H:2]1[C:3]([OH:5])=[O:4]

参加物

3

反応物 2

HCID 145742L-ProlineHCID 164533Alpha-Ketoglutarate

生成物 1

HCID 7098652cis-3-Hydroxy-L-proline

実験手順

The enzyme reaction using purified L-proline-3-hydroxylase obtained in Example 5 was carried out. The reaction mixture was composed of 200 mM TES buffer (pH 7.0), 20 mM L-proline, 20 mM 2-ketoglutarate, 5 mM L-ascorbic acids 1 mM, ferrous sulfate, and 106 U of purified enzyme preparation, the total volume being 100 μl. The reaction was carried out at 35° C. for 30 minutes. As a result of the reaction, 18 mM (2.4 g/l) of cis-3-hydroxy-L-proline was produced in the reaction mixture.

後処理

  1. customThe enzyme reaction
  2. customusing purified L-proline-3-hydroxylase
  3. customobtained in Example 5
  4. customAs a result of the reaction

出典と根拠

ORDレコード
ord-3d0b07d8c4af47af92d4d4afbfaf4ad0
出典データセット
uspto-grants-1999_10
特許番号
US05962292

関連ノート

読み込み中…