Sodium Carbonate → Biotin

HRID 1748841

反応式

HRID 1748841 の反応式
[CH2:1]1[S:5][C@@H:4]([CH2:6][CH2:7][CH2:8][CH2:9][C:10](NCCCCCC(ON2C(=O)C(S([O-])(=O)=O)CC2=O)=O)=[O:11])[C@H:3]2[NH:32][C:33]([NH:35][C@@H:2]12)=[O:34].[Na+].C(=O)([O-])[O-:38].[Na+].[Na+]>>[OH:38][C:10]([CH2:9][CH2:8][CH2:7][CH2:6][C@H:4]1[C@@H:3]2[C@@H:2]([NH:35][C:33]([NH:32]2)=[O:34])[CH2:1][S:5]1)=[O:11]

参加物

3

反応物 2

HCID 10340Sodium CarbonateHCID 71571497Sulphosuccinimidyl-6-(biotinamido)hexanoate

生成物 1

HCID 171548Biotin

実験手順

The RA-NAH (10 mg) obtained in the above was dissolved in 0.5 ml of 0.1 M sodium carbonate aqueous solution, subsequently adding 3 mg of sulfo-NHS-LC-biotin (mfd, by Pierce Chemical Company) thereto followed by overnight incubation at 4° C. After overnight dialysis against distilled water thereafter, the dialyzed liquid was lyophilized to obtain the polysaccharide of the present invention in which biotin is bound to the reducing end of NAH (to be referred to as “NAH-biotin” hereinafter) (8 mg).

後処理

  1. customThe RA-NAH (10 mg) obtained in the
  2. customat 4° C
  3. customAfter overnight dialysis
  4. distillationagainst distilled water

出典と根拠

ORDレコード
ord-1b26caafa6a644a48874c7472e44e884
出典データセット
uspto-grants-2015_10
特許番号
US09150902B2

関連ノート

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