[OH:1][C@@H:2]1[C@@H:6]([OH:7])[CH2:5][N:4]([C:8](=[O:18])[CH2:9][NH:10]C(=O)OC(C)(C)C)[C@@H:3]1[CH2:19][C:20]1[CH:25]=[CH:24][C:23]([O:26][CH3:27])=[CH:22][CH:21]=1.[F:28][C:29]([F:34])([F:33])[C:30]([OH:32])=[O:31]>C(Cl)Cl>[NH2:10][CH2:9][C:8]([N:4]1[CH2:5][C@H:6]([OH:7])[C@@H:2]([OH:1])[C@H:3]1[CH2:19][C:20]1[CH:25]=[CH:24][C:23]([O:26][CH3:27])=[CH:22][CH:21]=1)=[O:18].[C:30]([OH:32])([C:29]([F:34])([F:33])[F:28])=[O:31]Following the procedure as described in Example 11, except using material from Example 40 (350 mg, 0,920 mmol), CH2Cl2 (35 mL, trifluoroacetic acid (500 μlit, 6.49 mmol) and stirring at room temp for 45 min, the title compound is isolated (quantitative yield) as a mono TFA salt and is used crude, “as is”, in the next step. LC/MS (Condition A): ret. T=1.54 min, (M+H)+ 281.19.
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