Thymidine → Aflatoxin M1

HRID 1762967

反応式

HRID 1762967 の反応式
C1(N=C=N[CH:10]2[CH2:15][CH2:14][CH2:13][CH2:12][CH2:11]2)CCCCC1.OO.[C:18]([CH2:23][C:24]([O-:26])=O)([C:20]([OH:22])=O)=[O:19].N1[C:35](=[O:36])[C:34]2NC=N[C:30]=2N=C1.NNC1N[C:47](=[O:49])C2C(=NC=CN=2)N=1.[C@@H]1(N2[CH:66]=[C:64](C)C(=O)NC2=O)O[C@H](CO)[C@@H](O)C1.CC(CCCC(C)CCCC(C)CCCC(C)C)C.CC1(C)S[C@@H]2[C@H](NC(CC3C=CC=CC=3)=O)C(=O)N2[C@H]1[C:105]([O-:107])=[O:106].[K+].C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]2NC(N)=N)[C@H](O[C@@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@]1(O)C=O.C([O-])(=O)C(C)=O.[Na+].CCC1OC(C2C=CC(Cl)=CC=2Cl)(CN2N=CN=C2)OC1>O1CCCC1.CN(C)C=O>[CH3:47][O:49][C:10]1[CH:11]=[C:12]2[O:22][C@H:20]3[O:26][CH:24]=[CH:23][C@@:18]3([OH:19])[C:13]2=[C:14]2[C:15]=1[C:30]1[CH2:66][CH2:64][C:35](=[O:36])[C:34]=1[C:105]([O:107]2)=[O:106]

参加物

12

反応物 9

HCID 5789ThymidineHCID 15979PristaneHCID 91673EtaconazoleHCID 3260017Oxaloacetate IonHCID 20039623Hydrogen peroxide dicyclohexylcarbodiimideHCID 23662274Sodium pyruvateHCID 135398638HypoxanthineHCID 136025254Amino-pterinHCID 178431920HCID 178431920、化合物

生成物 1

HCID 15558498Aflatoxin M1

試薬・触媒・溶媒 2

HCID 6228Dimethylformamide溶媒HCID 8028Tetrahydrofuran溶媒

実験手順

Materials: All inorganic chemicals and organic solvents were of reagent grade or better. Bovine serum albumin (BSA) (fatty acid free and fraction V) ovalbumin (OA) (crude and fraction VII), polyethylene sorbitan monolaurate (Tween 20), 2,2'-azino-di-(3-ethylbentzthiazoline)sulfonic acid (ABTS), hydrogen peroxide dicyclohexylcarbodiimide, N-hydroxysuccidimide, dimethylformamide (DMF), polyethylene glycol (PEG) (MW 1450), insulin, oxaloacetate, hypoxanthine (H), amino-pterin (A), thymidine (T) and 2,6,10,14 tetramethylpentadecane (pristane), horseradish peroxidase (HRP) Type VI were purchased from Sigma Chemical Company (St. Louis, Mo.); tetrahydrofuran from Aldrich Chemical Company (Milwaukee, Wis.); Freund's complete and incomplete adjuvants from Difco (Detroit, Mich.); goat anti-mouse IgG conjugated to horseradish peroxidase from Cooper Biomedical (Malvern, Pa.); Dulbecco's Modified Eagle's Medium (DMEM), Penicillin/Streptomycin Solution (Pen/Strep) (100,000 U/ml), NCTC Medium 135, fetal bovine serum (FBS), and sodium pyruvate from Gibco Laboratories (Grand Island, N.Y.); microculture plates (96- and 24-well plates) from Costar (Cambridge, Mass.); microtiter plates from NUNC, Vangard International (Neptune, N.J.). Aflatoxins B1, B2, G1, G2 were purchased from Sigma Chemical Company (St. Louis, Mo.); aflatoxin M1 was generously provided by the Department of Botany and Plant Pathology, Michigan State University, East Lansing, Mich. Subclass identification kit was purchased from Boehringer Mannheim Biochemicals (Indianapolis, Ind.). The myeloma cell line P3/NS 1/1-Ag-4 (NS-1) (ATCC TIB 18) was purchased from The American Type Culture Collection (Rockville, Md.). Mice were purchased from Charles River Laboratories (Wilmington, Mass.).

出典と根拠

ORDレコード
ord-d964f58be0eb4db0bb995c3de33c36a3
出典データセット
uspto-grants-1989_05
特許番号
US04835100

関連ノート

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