Sodium Chloride → 2-Isobutyl-3-ketoglutarate diethyl ester

HRID 1793657

反応式

HRID 1793657 の反応式
P([O-])([O-])([O-])=O.[K+].[K+].[K+].[Na+].[Cl-].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[CH2:23]([O:25][C:26](=[O:41])[CH:27]([CH2:37][CH:38]([CH3:40])[CH3:39])[C:28](=[O:36])[C:29](=O)[C:30]([O:32][CH2:33][CH3:34])=[O:31])[CH3:24]>C(OCC)(=O)C.OCC(CO)O.CS(C)=O>[CH2:23]([O:25][C:26](=[O:41])[CH:27]([CH2:37][CH:38]([CH3:39])[CH3:40])[C:28](=[O:36])[CH2:29][C:30]([O:32][CH2:33][CH3:34])=[O:31])[CH3:24]

参加物

8

反応物 4

HCID 5234Sodium ChlorideHCID 62657Potassium Phosphate, TribasicHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2 回HCID 220229102-Isobutyl-3-ketoketoglutarate diethyl ester

生成物 1

HCID 99926592-Isobutyl-3-ketoglutarate diethyl ester

試薬・触媒・溶媒 3

HCID 679Dimethyl Sulfoxide溶媒HCID 753Glycerol溶媒HCID 8857Ethyl Acetate溶媒

実験手順

To 40 mL of 300 mM potassium phosphate buffer buffer, pH=6.5, containing NaCl (100 mM), DMSO (3% v:v), glucose (200 mM), and glycerol (10% v:v), 40 mM of 2-isobutyl-3-ketoketoglutarate diethyl ester was added along with 100 mg of lyophilized KRED 1001 and 30 mg of glucose dehydrogenase. The reaction mixture was incubated for 48 hours at 37 C. Gas chromatographic analysis showed that the yield of hydroxy diester product was greater than 80%. The product was isolated by extraction of the reaction mixture with ethyl acetate and purification using silica gel chromatography. Isolated yield was in the range of 85–95%.

後処理

  1. customIsolated
  2. customyield

出典と根拠

ORDレコード
ord-cb2716e4893e47f5b420d1a47f407af7
出典データセット
uspto-grants-2006_07
特許番号
US07081535B2

関連ノート

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