Diisopropylethylamine → 5-(3-((2-(1,2,4-oxadiazol-3-yl)propan-2-yl)carbamoyl)-4-fluorophenyl)-2-(4-fluorophenyl)-N-methyl-6-((2,2,2-trifluoroethyl)amino)furo[2,3-b]pyridine-3-carboxamide

2-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)-1,1,3,3-tetramethylisouronium hexafluorophosphate(V) (20 mg, 0.053 mmol) was added to stirring solution of 2-fluoro-5-(2-(4-fluorophenyl)-3-(methylcarbamoyl)-6-((2,2,2-trifluoroethyl)amino)furo[2,3-b]pyridin-5-yl)benzoic acid (18 mg, 0.036 mmol), N-ethyl-N-isopropylpropan-2-amine (0.019 mL, 0.11 mmol) and 2-(1,2,4-oxadiazol-3-yl)propan-2-amine hydrochloride (7.6 mg, 0.046 mmol) in DMF (1 mL) at rt. The mixture was allowed to stir at rt. for 30 min. The crude reaction mixture was purified via preparative LC/MS with the following conditions: Column:Waters XBridge C18, 19×200 mm, 5-μm particles; Mobile Phase A: water with 20-mM ammonium acetate; Mobile Phase B: 95:5 acetonitrile:water with 20-mM ammonium acetate; Gradient: 30-70% B over 20 minutes, then a 5-minute hold at 100% B; Flow: 20 mL/min. Fractions containing the desired product were combined and dried via centrifugal evaporation. The yield of the product was 15.9 mg, and its estimated purity by LCMS analysis was 100%. Two analytical LC/MS injections were used to determine the final purity. Injection 1 conditions: Column:Waters BEH C18, 2.0×50 mm, 1.7-μm particles; Mobile Phase A: 5:95 acetonitrile:water with 10 mM ammonium acetate; Mobile Phase B: 95:5 acetonitrile:water with 10 mM ammonium acetate; Temperature: 40° C.; Gradient: 0.5 min hold at 0% B, 0-100% B over 4 minutes, then a 0.5-minute hold at 100% B; Flow: 1 mL/min. retention time: 2.92 min; M+H: 615. Injection 2 conditions: Column:Waters BEH C18, 2.0×50 mm, 1.7-μm particles; Mobile Phase A: 5:95 methanol:water with 10 mM ammonium acetate; MobilePhase B: 95:5 methanol:water with 10 mM ammonium acetate; Temperature: 40° C.; Gradient: 0.5 min hold at 0% B, 0-100% B over 4 minutes, then a 0.5-minute hold at 100% B; Flow: 0.5 mL/min. retention time: 3.91 min; M+H: 615. Proton NMR was acquired in deuterated DMSO. 1H NMR (500 MHz, DMSO-d6) δ 9.47 (s, 1H), 8.73 (s, 1H), 8.41-8.34 (m, 1H), 8.00-7.92 (m, 2H), 7.68 (s, 1H), 7.64-7.54 (m, 2H), 7.44 (t, J=9.2 Hz, 1H), 7.35 (t, J=9.0 Hz, 2H), 6.70 (t, J=6.4 Hz, 1H), 4.24-4.13 (m, 2H), 2.80 (d, J=4.6 Hz, 3H), 1.69 (s, 6H).

1. customThe crude reaction mixture
2. customwas purified via preparative LC/MS with the following conditions
3. waitGradient: 30-70% B over 20 minutes
4. customa 5-minute hold
5. additionFractions containing the desired product
6. customdried via centrifugal evaporation
7. custom40° C.
8. waitGradient: 0.5 min hold at 0% B, 0-100% B over 4 minutes
9. customa 0.5-minute hold
10. custom40° C.
11. waitGradient: 0.5 min hold at 0% B, 0-100% B over 4 minutes
12. customa 0.5-minute hold

ORDレコード

ord-25655cb99b7b43a0b61fc6a6d813898d

出典データセット

uspto-grants-2015_02

DOI

10.6084/m9.figshare.5104873.v1

特許番号

US08962651B2