Tetrasodium Pyrophosphate → Calcium Pyrophosphate Dihydrate
反応式
[Cl-].[Ca+2:2].[Cl-].[O-:4][P:5]([O:8][P:9]([O-:12])([O-:11])=[O:10])(=[O:7])[O-:6].[Na+].[Na+].[Na+].[Na+].[Ca].[P]>>[OH2:4].[OH2:4].[O-:6][P:5]([O:8][P:9]([O-:12])([O-:11])=[O:10])(=[O:4])[O-:7].[Ca+2:2].[Ca+2:2]参加物
4
実験手順
An aqueous calcium chloride solution and an aqueous sodium pyrophosphate solution were mixed together so that the calcium/phosphorus atomic ratio became 1:1, and the mixture was allowed to stand for a whole day to form calcium diphosphate dihydrate (Ca2P2O7.2H2O) crystals, which were then filtered off. The thus prepared material was packed into a column in the same manner as in Example 1, and a mixed sample of serum albumin, lysozyme and cytochrome c was chromatographed thereon. As a result the serum albumin flowed through the column without being retained therein, while the lysozyme and cytochrome c were retained therein.