A solution of 2-(benzylamino)-4-(4-benzoylpiperazin-1-yl)benzoic acid (80 mg, 0.2 mmol), 2 M ammonia ethanol solution (0.60 ml, 1.2 mmol), tiethylamine (0.08 ml, 0.57 mmol) and diphenylphosphoryl azide (0.045 ml, 0.2 mmol) in 15 ml THF was stirred for 16 hours. The run was quenched with 1 ml water and all the solvents were evaporated under vacuum. 2-(benzylamino)-4-(4-bezoylpiperazin-1-yl)benzamide (40 mg, 40%) was obtained by flash column chromatograph (60% ethyl acetate in hexanes), and its hydrochloride was made by bubbling HCL gas in ether solution. 1H NMR (500 MHz, DMSO-d6) δ (ppm) 3.28-3.670 (br, 8H), 4.43 (s, 2H), 5.00 (s, 1H), 6.06 (s, 1H), 6.24 (d, J=8.2 Hz, 1H), 7.00 (s, 1H), 7.12-7.65 (m, 13H), 7.67 (d, J=8.8 Hz, 1H). 13C NMR (125 MHz, DMSO-d6): δ (ppm) 43.5, 46.0, 95.3, 102.7, 127.0, 127.3, 128.3, 128.4, 128.5, 128.6, 129.6, 132.4, 136.0, 139.3, 151.7, 154.2, 167.0, 168.9.
