[Cl:1][C:2]1[N:12]([C:13]2[CH:18]=[CH:17][CH:16]=[CH:15][CH:14]=2)[C:5]2=[CH:6][N:7]=[C:8]([O:10][CH3:11])[CH:9]=[C:4]2[C:3]=1[C:19](O)=[O:20].[N:22]1([C:28]([O:30][C:31]([CH3:34])([CH3:33])[CH3:32])=[O:29])[CH2:27][CH2:26][NH:25][CH2:24][CH2:23]1.Cl.C(N=C=NCCCN(C)C)C.O.ON1C2C=CC=CC=2N=N1.CN1CCOCC1>CN(C=O)C>[C:31]([O:30][C:28]([N:22]1[CH2:27][CH2:26][N:25]([C:19]([C:3]2[C:4]3[C:5](=[CH:6][N:7]=[C:8]([O:10][CH3:11])[CH:9]=3)[N:12]([C:13]3[CH:18]=[CH:17][CH:16]=[CH:15][CH:14]=3)[C:2]=2[Cl:1])=[O:20])[CH2:24][CH2:23]1)=[O:29])([CH3:34])([CH3:32])[CH3:33]To a solution of the crude compound of step 4, tert-butyl 1-piperazinecarboxylate (565 mg, 3.03 mmol), N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride (581 mg, 3.03 mmol) and 1-hydroxybenzotriazole hydrate (312 mg, 2.29 mmol) in DMF (20 ml) was added NMM (911 μl, 8.27 mmol), and the reaction mixture was stirred at room temperature overnight. The mixture was quenched with water and extracted with EA. The organic layer was separated, dried over sodium sulfate, filtered and evaporated.
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