2-Mercaptoethanol → (S)-2-amino-4-(methylthio)butane-1-sulfonic acid hydrochloride

HRID 2040509ノート

反応式

HRID 2040509 の反応式
C1N(CCO)CC[N:3]([CH2:10][CH2:11][S:12]([OH:15])(=[O:14])=[O:13])C1.[Mg+2].[Cl-:17].[Cl-].[Cl-].[K+].[SH:21][CH2:22][CH2:23]O.P(O[CH2:38][C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O>OCC(CO)O>[ClH:17].[NH2:3][C@@H:10]([CH2:23][CH2:22][S:21][CH3:38])[CH2:11][S:12]([OH:15])(=[O:14])=[O:13]

参加物

7

反応物 5

HCID 15672-MercaptoethanolHCID 4873Potassium ChlorideHCID 59575'-AtpHCID 238314-(2-Hydroxyethyl)-1-piperazineethanesulfonic acidHCID 5360315Magnesium Chloride

生成物 1

HCID 86656462(S)-2-amino-4-(methylthio)butane-1-sulfonic acid hydrochloride

試薬・触媒・溶媒 1

HCID 753Glycerol溶媒

実験手順

The assay system is as described by Hartman M et al, 2006 Enzymatic aminoacylation of tRNA with unnatural amino acids. PNAS. 103(12):4356-4361 (the content of which is hereby incorporated by reference, particularly with respect to the experimental methods described therein). Each assay contains 40 mM Hepes (pH 7.4), 17 mM MgCl2, 45 mM KCl, 3.4 mM 2-mercaptoethanol, 6 mM ATP, 6% glycerol, 350 μM E. coli tRNA (Roche), 0.09 mg/mL BSA, 910 nM Methionyl-tRNA synthetase (MetRS) and amino acids in 100-1,000 μM range. The assay is initiated by addition of the mixture of AARS and incubated at 25-30° C. for 10 minutes. Samples are transferred to ice bath to stop the reactions and then small aliquot (2 μL) samples are precipitated using 500 μL of 5% cold TCA onto Whatmann filter papers and vacuumed and then dried under a heat lamp. Radioactivity of each sample is measured as counts per minute (CPM), as compared to controls (without the analog). Results for the S enantiomer of I-A ((S)-2-amino-4-(methylthio)butane-1-sulfonic acid hydrochloride) are shown below in Table 11.

後処理

  1. additionThe assay is initiated by addition of the mixture of AARS
  2. customSamples are transferred to ice bath
  3. customsmall aliquot (2 μL) samples are precipitated
  4. filtrationfilter papers
  5. customdried under a heat lamp

出典と根拠

ORDレコード
ord-2eaaa0a14d1c43989f9b6ee57441060a
出典データセット
uspto-grants-2013_11
特許番号
US08580859B2

反応ノート

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