Acetate → HCID 86638818、化合物

HRID 2090135ノート

反応式

HRID 2090135 の反応式
[C:1]([O-:4])(=[O:3])[CH3:2].[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[Na+:10].[P:11]([O-:15])([OH:14])([OH:13])=[O:12].[K+:16]>O>[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[K+:16].[Na+:10].[P:11]([O-:15])([OH:14])([OH:13])=[O:12].[C:1]([O-:4])(=[O:3])[CH3:2].[Na+:10]

参加物

5

反応物 3

HCID 175Acetate3 回HCID 516951Monobasic potassium phosphate3 回HCID 23672064Monosodium phosphate3 回

生成物 1

HCID 86638818HCID 86638818、化合物

試薬・触媒・溶媒 1

HCID 962Water溶媒

実験手順

Batch crystallization was performed by admixing around 400 μL of each protein solution with an equal amount of crystallization solution in a 1.5 mL Eppendorff reaction tube. 400 μL of a particular crystallization solution was prepared by admixing acetate buffer, sodium dihydrogen phosphate stock solution, potassium dihydrogen phosphate stock solution and Milli Q water. In this example, the acetate buffer molarity was 0.1 M, and the acetate buffer pH was around 4.1. The following combination of sodium dihydrogen phosphate and potassium dihydrogen phosphate was used: around 0.9 M sodium dihydrogen phosphate and around 0.9 M potassium dihydrogen phosphate. The reaction tubes were stored at ambient temperature. Microscopy of 1 μL aliquots was performed after 11 days.

後処理

  1. customBatch crystallization
  2. customwith an equal amount of crystallization solution in a 1.5 mL
  3. customEppendorff reaction tube
  4. customThe reaction tubes
  5. customwere stored at ambient temperature

出典と根拠

ORDレコード
ord-b09a058af0b042729bff9147ac1eab80
出典データセット
uspto-grants-2013_05
特許番号
US08436149B2

反応ノート

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