反応式
[C:1]([O-:9])(=[O:8])[CH:2]([CH2:4][C:5]([O-:7])=[O:6])[OH:3].[Na+].[Na+]>O>[C:1]([OH:9])(=[O:8])[CH:2]([CH2:4][C:5]([OH:7])=[O:6])[OH:3]参加物
試薬・触媒・溶媒 1
実験手順
The following solutions were prepared: 1 μL of 1M potassium ferricyanide aqueous solution obtained by dissolving potassium ferricyanide (manufactured by Wako Pure Chemical Industries, Ltd.) in pure water; 6.7 μL of 1000 U/mL diaphorase solution obtained by dissolving diaphorase (manufactured by UNITIKA LTD.) in 50 mM phosphate buffer (pH 7.5); 7.8 μL of 4M sodium malate aqueous solution obtained by dissolving sodium malate (manufactured by Wako Pure Chemical Industries, Ltd.) in pure water; 7.8 μL of 4M malic acid aqueous solution obtained by dissolving malic acid (manufactured by Wako Pure Chemical Industries, Ltd.) in pure water; 7.8 μL of 4M potassium malate aqueous solution obtained by dissolving potassium malate (manufactured by KANTO CHEMICAL CO., INC.) in pure water; 1 μL of 5 mM NADP aqueous solution obtained by dissolving NADP (manufactured by ORIENTAL YEAST Co., Ltd.) in pure water; and 1 μL of 25000 U/mL malate dehydrogenase solution containing malate dehydrogenase dissolved in 50% of glycerol (manufactured by Roche Diagnostics, Inc.).
出典と根拠
- ORDレコード
- ord-8a8b58189cdb4263896b7ed368d3e206
- 出典データセット
- uspto-grants-2009_04
- 特許番号
- US07521214B2
関連ノート
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