Dimethyl Sulfoxide → D-Galactose

HRID 2172376

反応式

HRID 2172376 の反応式
CS(C)=[O:3].[OH:5][CH2:6][C:7]1([O:16][CH2:15][C@@H:13]([OH:14])[C@@H:11]([OH:12])[C@@H:9]1[OH:10])[OH:8].[NH2:17][C@H:18]([C:24]([OH:26])=[O:25])[CH2:19][CH2:20][C:21](=[O:23])[OH:22]>O.C(=O)([O-])[O-]>[NH2:17][C@H:18]([C:24]([OH:26])=[O:25])[CH2:19][CH2:20][C:21](=[O:22])[OH:23].[OH:5][CH2:6][C:7]1([O:16][CH2:15][C@@H:13]([OH:14])[C@@H:11]([OH:12])[C@@H:9]1[OH:10])[OH:8].[OH:3][CH:15]1[O:16][C@H:7]([CH2:6][OH:5])[C@H:9]([OH:10])[C@H:11]([OH:12])[C@H:13]1[OH:14].[OH:22][CH:15]1[O:16][C@H:7]([CH2:6][OH:5])[C@@H:9]([OH:10])[C@H:11]([OH:12])[C@@H:13]1[OH:14]

参加物

9

反応物 3

HCID 679Dimethyl Sulfoxide2 回HCID 33032L-Glutamic Acid3 回HCID 2723872D-Fructose2 回

生成物 4

HCID 6036D-GalactoseHCID 18950D-MannoseHCID 33032L-Glutamic AcidHCID 2723872D-Fructose

試薬・触媒・溶媒 2

HCID 962Water溶媒HCID 19660Carbonate Ion溶媒2 回

実験手順

Solutions of Glu (40 mM-4.0 μM), Fru (0.4 mM), Gal (0.4 mM) and Man (0.4 mM) in deionized water were prepared. An aliquot (2.0 mL) of the solution of TPEDB (0.1 mM) in the carbonate buffer containing 4 vol % DMSO was added into a 10 mL volumetric flask, followed by the addition of 1.0 mL of a solution of Fru (0.4 mM) and 1.0 mL of a solution of Glu (40 mM). After shaking for a few seconds, a solution of TPEDB (50 μM) with 10 mM of Glu and 0.1 mM of Fru in the carbonate buffer containing 2% DMSO was obtained. The solution was allowed to stand for 30 min before its FL measurement. The solution of TPEDB and Glu containing another saccharide (Gal or Man) was prepared by similar procedures.

後処理

  1. additionwas added into a 10 mL volumetric flask
  2. customwas obtained

出典と根拠

ORDレコード
ord-a1162ef75de54e14a34af4a42eb8f286
出典データセット
uspto-grants-2015_06
特許番号
US09051598B2

関連ノート

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