関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | Potassium Chloride | HCID 4873 | — |
| 反応物 | Sodium Chloride | HCID 5234 | — |
| 反応物 | Egta | HCID 6207 | — |
| 反応物 | Potassium Hydroxide | HCID 14797 | — |
| 反応物 | 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | HCID 23831 | — |
| 反応物 | Cesium Chloride | HCID 24293 | — |
| 反応物 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| 反応物 | Calcium Chloride | HCID 5284359 | — |
| 反応物 | Magnesium Chloride | HCID 5360315 | — |
| 反応物 | Gluconate | HCID 6419706 | — |
| 反応物 | QX-314 bromide | HCID 9884487 | — |
| 生成物 | X-Gal | HCID 65181 | — |
反応手順
Electrophysiology. Cells were analyzed at indicated time points after dox induction. Action potentials were recorded with current-clamp whole-cell configuration. The pipette solution for current-clamp experiments contained (in mM) 123 K-gluconate, 10 KCl, 1 MgCl2, 10 HEPES, 1 EGTA, 0.1 CaCl2, 1 K2ATP, 0.2 Na4GTP and 4 glucose, pH adjusted to 7.2 with KOH. Membrane potentials were kept around −65 to −70 mV, and step currents were injected to elicit action potentials. For voltage-clamp experiments, the internal solution contained (in mM) CsCl 135, HEPES 10, EGTA 1, Mg-ATP 4, Na4GTP 0.4, and QX-314 10, pH7.4. The bath solution contained (in mM): NaCl 140, KCl 5, CaCl2 2, MgCl2 2, HEPES 10, and glucose 10, pH7.4. Whole-cell currents including Na+ and K+ currents were recorded at a holding potential of −70 mV, voltage steps ranging from −80 mV to +90 mV were delivered at 10 mV increments.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US09057053B2 |
|---|
| 出典データセット | uspto-grants-2015_06 |
|---|
| ORD ID | ord-8c17c882d8d64bd6bd87b6e5079851fa |
|---|
| 出典メモ | uspto-grants-2015_06 |
|---|
