反应详情

Electrophysiology. Cells were analyzed at indicated time points after dox induction. Action potentials were recorded with current-clamp whole-cell configuration. The pipette solution for current-clamp experiments contained (in mM) 123 K-gluconate, 10 KCl, 1 MgCl2, 10 HEPES, 1 EGTA, 0.1 CaCl2, 1 K2ATP, 0.2 Na4GTP and 4 glucose, pH adjusted to 7.2 with KOH. Membrane potentials were kept around −65 to −70 mV, and step currents were injected to elicit action potentials. For voltage-clamp experiments, the internal solution contained (in mM) CsCl 135, HEPES 10, EGTA 1, Mg-ATP 4, Na4GTP 0.4, and QX-314 10, pH7.4. The bath solution contained (in mM): NaCl 140, KCl 5, CaCl2 2, MgCl2 2, HEPES 10, and glucose 10, pH7.4. Whole-cell currents including Na+ and K+ currents were recorded at a holding potential of −70 mV, voltage steps ranging from −80 mV to +90 mV were delivered at 10 mV increments.