Phosphoric Acid → Benzoic Acid

HRID 2211083ノート

反応式

HRID 2211083 の反応式
CN1[C@H]2[C@@H](C(OC)=O)[C@@H]([O:10][C:11]([C:13]3[CH:14]=[CH:15][CH:16]=[CH:17][CH:18]=3)=[O:12])C[C@@H]1CC2.CN1[C@H]2[C@@H](C(OC)=O)[C@@H](OC(C3C=CC=CC=3)=O)C[C@@H]1CC2.Cl.P(=O)(O)(O)O>C1N(CCCS(O)(=O)=O)CCOC1>[C:11]([OH:12])(=[O:10])[C:13]1[CH:14]=[CH:15][CH:16]=[CH:17][CH:18]=1

参加物

5

反応物 3

HCID 1004Phosphoric AcidHCID 446220CocaineHCID 656832(-)-Cocaine hydrochloride

生成物 1

HCID 243Benzoic Acid

試薬・触媒・溶媒 1

HCID 70807Mops溶媒

実験手順

Cocaine esterase in extracts, column fractions and characterisation experiments (e.g. pH optimum, Michaelis constant determinations) was routinely assayed by shaking incubation (250 rpm, 30° C.) of 2 mM cocaine HCl (made up in 1 ml 50 mM MOPS buffer, pH 7.0) with a known quantity of sample (10-200 μl, 0-0.01 U) for a fixed length of time (10-30 min). The reaction was stopped by the addition of 10 μl of concentrated phosphoric acid and protein pelleted at 13,000 rpm in a minifuge. The amount of benzoic acid produced was determined by HPLC analysis of 50 μl of the supernatant and comparison to standard samples (0-1 mM) treated identically. Standard curves of peak height plotted against benzoic acid concentration were linear over the range 0-1 mM. Controls containing no enzyme solution were run to allow for background hydrolysis. An assay was considered invalid if no cocaine remained at the end of the assay (seen by HPLC). One unit of esterase activity is defined as the amount of enzyme required to produce 1 μmol of benzoic acid in 1 min at 30° C.

後処理

  1. customfor a fixed length of time (10-30 min)

出典と根拠

ORDレコード
ord-cf6375f79c7b4b8b81b8eda42eb538b3
出典データセット
uspto-grants-1995_10
特許番号
US05462868

反応ノート

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