NHS-Biotin → Biotin

HRID 22628

反応式

HRID 22628 の反応式
C1C(=O)N([O:8][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][CH:15]2[S:19][CH2:18][CH:17]3[NH:20][C:21]([NH:23][CH:16]23)=[O:22])=[O:10])C(=O)C1>C(=O)([O-])O.[Na+]>[OH:10][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][C@H:15]1[C@@H:16]2[C@@H:17]([NH:20][C:21]([NH:23]2)=[O:22])[CH2:18][S:19]1)=[O:8]

参加物

3

反応物 1

HCID 434213NHS-Biotin

生成物 1

HCID 171548Biotin

試薬・触媒・溶媒 1

HCID 516892Sodium Bicarbonate溶媒

実験手順

Each 3-mg portion of the 32-clone monoclonal antibodies prepared in (1) was mixed with 10 mg of biotinyl N-hydroxysuccinimide ester (product of Zymed) and the reaction was carried out in 0.1 M sodium hydrogen carbonate (pH 8) with stirring at room temperature for 3 hours. The reaction mixture was dialyzed overnight against 5 L of PBS at 4° C. to give the biotin-labeled monoclonal antibody.

後処理

  1. customThe reaction mixture was dialyzed overnight against 5 L of PBS at 4° C.

出典と根拠

ORDレコード
ord-53d572a8e9d04798981f64c307b0a0f5
出典データセット
uspto-grants-2005_02
特許番号
US06849419B1

関連ノート

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